Misiorek Maria, Sekuła Justyna, Ruman Tomasz
Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave., 35-959, Rzeszów, Poland.
Phytochem Anal. 2017 Nov;28(6):479-486. doi: 10.1002/pca.2696. Epub 2017 Jun 13.
Garlic (Allium sativum) is the subject of many studies due to its numerous beneficial properties. Although compounds of garlic have been studied by various analytical methods, their tissue distributions are still unclear. Mass spectrometry imaging (MSI) appears to be a very powerful tool for the identification of the localisation of compounds within a garlic clove.
Visualisation of the spatial distribution of garlic low-molecular weight compounds with nanoparticle-based MSI.
Compounds occurring on the cross-section of sprouted garlic has been transferred to gold-nanoparticle enhanced target (AuNPET) by imprinting. The imprint was then subjected to MSI analysis.
The results suggest that low molecular weight compounds, such as amino acids, dipeptides, fatty acids, organosulphur and organoselenium compounds are distributed within the garlic clove in a characteristic manner. It can be connected with their biological functions and metabolic properties in the plant.
New methodology for the visualisation of low molecular weight compounds allowed a correlation to be made between their spatial distribution within a sprouted garlic clove and their biological function. Copyright © 2017 John Wiley & Sons, Ltd.
大蒜（Allium sativum）因其众多有益特性而成为许多研究的对象。尽管大蒜的化合物已通过各种分析方法进行了研究，但其组织分布仍不清楚。质谱成像（MSI）似乎是一种非常强大的工具，可用于识别大蒜蒜瓣内化合物的定位。
通过基于纳米颗粒的MSI可视化大蒜低分子量化合物的空间分布。
通过印记将发芽大蒜横截面上出现的化合物转移到金纳米颗粒增强靶标（AuNPET）上。然后对印记进行MSI分析。
结果表明，低分子量化合物，如氨基酸、二肽、脂肪酸、有机硫和有机硒化合物，以一种特征性方式分布在大蒜蒜瓣内。这可能与其在植物中的生物学功能和代谢特性有关。
用于可视化低分子量化合物的新方法使我们能够在发芽大蒜蒜瓣内它们的空间分布与其生物学功能之间建立关联。版权所有© 2017约翰威立国际出版公司。
