Xu Wen-Li, Zhang Qin, Huang Cheng-Jun, Lan Ya-Jia, Wang Yong-Wei, Xiong Lin-Sen
Department of Environmental Health and Occupational Medicine, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Shanghai Pudong New Area Center for Disease Control and Prevention, Shanghai 200120, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Jan;48(1):86-90.
To investigate the polymorphisms in interleukin 17A and interleukin 17F () and their relationship with pulmonary inflammation risk of dust exposed workers.
A case-control study among 193 subjects, including 67 subjects in case group and 126 in control group was conducted. PCR-RFLP was applied to genotype (G-197A) and (7488T/C). Logistic regression analysis was used to determine the effects of (G-197A) and (7488T/C) on the lung inflammation risk in dust exposed workers.
The genotypes analysis showed that the proportions of (G-197A) A/A, A/G and G/G were 42 (21.76%), 95 (49.22%), 56 (29.02%) in 193 cases, respectively, and the (7488T/C) T/T, T/C and C/C genotypes were 128 (66.32%), 54 (28.98%), 11 (5.70%), respectively. The frequency distribution of each genotype was consistent with the Hardy-Weinberg equilibrium fixed law. The ratio of (G-197A) A/A in the case group was lower than that of control group ( <0.05), while the G/G and A/G genotypes were higher than that of control group ( <0.05). Furthermore, the genotypes of (G-197A) A/G (=5.03, <0.01) and G/G(=3.35, <0.05) were associated with an increased risk of lung inflammation in workers exposed to dust. The frequency distribution difference of (7488T/C) T/T, T/C and C/C genotypes in the cases and control group was unobvious ( >0.05).
Under the same dust concentration, the dust exposed workers carrying (G-197A) A/G, G/G genotypes are more susceptible to pulmonary inflammation in the southwest of China.
研究白细胞介素17A（IL-17A）和白细胞介素17F（IL-17F）基因多态性及其与粉尘暴露工人肺部炎症风险的关系。
对193名受试者进行病例对照研究，其中病例组67例，对照组126例。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对IL-17A（G-197A）和IL-17F（7488T/C）进行基因分型。采用Logistic回归分析确定IL-17A（G-197A）和IL-17F（7488T/C）对粉尘暴露工人肺部炎症风险的影响。
基因分型分析显示，193例患者中IL-17A（G-197A）的A/A、A/G和G/G基因型比例分别为42例（21.76%）、95例（49.22%）、56例（29.02%），IL-17F（7488T/C）的T/T、T/C和C/C基因型分别为128例（66.32%）、54例（28.98%）、11例（5.70%）。各基因型频率分布符合Hardy-Weinberg平衡定律。病例组IL-17A（G-197A）的A/A基因型比例低于对照组（P<0.05），而G/G和A/G基因型高于对照组（P<0.05）。此外，IL-17A（G-197A）的A/G基因型（OR=5.03，P<0.01）和G/G基因型（OR=3.35，P<0.05）与粉尘暴露工人肺部炎症风险增加相关。病例组和对照组IL-17F（7488T/C）的T/T、T/C和C/C基因型频率分布差异不明显（P>0.05）。
在相同粉尘浓度下，携带IL-17A（G-197A）A/G、G/G基因型的中国西南部粉尘暴露工人更易患肺部炎症。
