Arisawa Tomiyasu, Tahara Tomomitsu, Shibata Tomoyuki, Nagasaka Mitsuo, Nakamura Masakatsu, Kamiya Yoshio, Fujita Hiroshi, Nakamura Masahiko, Yoshioka Daisuke, Arima Yuko, Okubo Masaaki, Hirata Ichiro, Nakano Hiroshi
Department of Gastroenterology, Fujita Health University School of Medicine, 1-98, Dengakugakubo, Kutsukake-cho, Toyoake 470-1192, Japan.
J Clin Immunol. 2008 Jan;28(1):44-9. doi: 10.1007/s10875-007-9125-8. Epub 2007 Sep 9.
We investigated the association between ulcerative colitis (UC) and polymorphisms of IL-17A (rs2275913, G-197A) and IL-17F (rs763780, 7488T/C) genes. We employed the multiplex PCR-SSCP method to detect gene polymorphisms. Both the numbers of -197A (IL-17A) and 7488T (IL-17F) alleles were significantly correlated to the development of UC. The frequencies of -197A/A and 7488T/T genotypes in the UC group were significantly higher than those in the non-UC group. An adjusted analysis revealed that -197A and 7488T alleles were independent risk factors for the developing UC. In addition, both polymorphisms were significantly associated with the pancolitis phenotype. Furthermore, -197A allele was significantly correlated to the chronic relapsing phenotype and -197A/A homozygote was more frequent in steroid-dependent cases, whereas 7488T allele was correlated with the chronic continuous phenotype. Our results provided the first evidence that -197A (IL-17A) and 7488T (IL-17F) alleles may influence the susceptibility to and pathophysiological features of UC independently.
我们研究了溃疡性结肠炎（UC）与白细胞介素-17A（IL-17A，rs2275913，G-197A）和白细胞介素-17F（IL-17F，rs763780，7488T/C）基因多态性之间的关联。我们采用多重聚合酶链反应-单链构象多态性（PCR-SSCP）方法检测基因多态性。-197A（IL-17A）和7488T（IL-17F）等位基因的数量均与UC的发生显著相关。UC组中-197A/A和7488T/T基因型的频率显著高于非UC组。校正分析显示，-197A和7488T等位基因是UC发生的独立危险因素。此外，两种多态性均与全结肠炎表型显著相关。此外，-197A等位基因与慢性复发表型显著相关，-197A/A纯合子在类固醇依赖病例中更为常见，而7488T等位基因与慢性持续表型相关。我们的结果首次证明，-197A（IL-17A）和7488T（IL-17F）等位基因可能独立影响UC的易感性和病理生理特征。
