Loren Pía, Sánchez Raúl, Arias María-Elena, Felmer Ricardo, Risopatrón Jennie, Cheuquemán Carolina
Centro de Biotecnología de la Reproducción (BIOREN-CEBIOR), Facultad de Medicina, Universidad de La Frontera, Temuco 4811230, Chile.
Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco 4811230, Chile.
Int J Mol Sci. 2017 Jun 14;18(6):1119. doi: 10.3390/ijms18061119.
Oxidative and nitrosative stress are common problems when handling gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because their absence or overproduction causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during in vitro fertilization (IVF) has deleterious or positive effects, depending on the concentration used in the culture medium, demonstrating the delicate balance between antioxidant and pro-oxidant activity. Further research is needed to better understand the possible impact of melatonin on the different IVP steps in humans and other mammals, especially in seasonal breeds where this neuro-hormone system highly regulates its reproduction physiology.
在体外处理配子时，氧化应激和亚硝化应激是常见问题。哺乳动物胚胎的体外发育受到培养条件的高度影响，尤其是活性氧（ROS）和活性氮（RNS），因为它们的缺乏或过量产生会导致胚胎发育停滞和基因表达变化。在体外受精（IVF）过程中配子共同孵育时，褪黑素根据培养基中使用的浓度具有有害或积极作用，这表明抗氧化和促氧化活性之间存在微妙的平衡。需要进一步研究以更好地了解褪黑素对人类和其他哺乳动物不同体外生产步骤的可能影响，特别是在季节性繁殖动物中，这种神经激素系统高度调节其生殖生理。
