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褪黑素与左旋肉碱联合应用对小鼠卵母细胞体外成熟的影响：一项实验研究。

Effects of combination of melatonin and L-carnitine on in vitro maturation in mouse oocytes: An experimental study.

作者信息

Chegini Raziye, Sadeghi Morteza, Shirian Sadegh, Sabbaghziarani Fatemeh, Aali Ehsan, Soleimani Pouriya, Reza Ashtari Majelan Mohammad, Zafari Fariba, Darabi Shahram

机构信息

Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

出版信息

Int J Reprod Biomed. 2024 Sep 12;22(7):527-538. doi: 10.18502/ijrm.v22i7.16961. eCollection 2024 Jul.

DOI:10.18502/ijrm.v22i7.16961
PMID:39355312
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11441286/
Abstract

BACKGROUND

Melatonin and L-carnitine are free radical scavengers with antiapoptotic and antioxidant properties that improve oocyte development.

OBJECTIVE

This study aimed to find the possible effect of combining 2 antioxidant agents of melatonin and L-carnitine on oocyte morphology, maturation, apoptosis, and expression of bone morphogenetic protein 15 () and growth differentiation factor 9 () genes in a mice model.

MATERIALS AND METHODS

To overstimulation, 60 female NMRI mice were injected intraperitoneally using mare serum gonadotropin. On day 2 post-injection, 70 cumulus-oocyte complexes were collected from each mouse. The collected oocytes randomly were then divided into 4 groups including, the control, melatonin, L-carnitine, and melatonin + L-carnitine groups. The morphology and maturation rate of the oocytes was evaluated using a light microscope. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and the expression of and growth and differentiation factor genes was also evaluated by real-time polymerase chain reaction.

RESULTS

Oocyte diameter significantly was increased in combination treatment of L-carnitine and melatonin compared to other groups (p 0.05). L-carnitine group showed the highest mean percentage of oocyte cytoplasmic pattern. Results of the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling indicated that the lowest apoptosis rate belonged to the melatonin + L-carnitine group. Moreover, the combination groups showed the highest number of oocytes and maturation rate. The and genes were significantly upregulated in all treatment groups compared to the control group.

CONCLUSION

Our results suggested a combination of melatonin + L-carnitine administration as a more effective choice for in vitro promotion of oocyte maturation.

摘要

背景

褪黑素和左旋肉碱是具有抗凋亡和抗氧化特性的自由基清除剂，可改善卵母细胞发育。

目的

本研究旨在探讨联合使用褪黑素和左旋肉碱这两种抗氧化剂对小鼠模型中卵母细胞形态、成熟、凋亡以及骨形态发生蛋白15（BMP15）和生长分化因子9（GDF9）基因表达的可能影响。

材料与方法

为进行超促排卵，给60只雌性NMRI小鼠腹腔注射马血清促性腺激素。注射后第2天，从每只小鼠收集70个卵丘 - 卵母细胞复合体。然后将收集到的卵母细胞随机分为4组，包括对照组、褪黑素组、左旋肉碱组和褪黑素 + 左旋肉碱组。使用光学显微镜评估卵母细胞的形态和成熟率。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法鉴定凋亡情况，并通过实时聚合酶链反应评估BMP15和生长分化因子9基因的表达。

结果

与其他组相比，左旋肉碱和褪黑素联合处理组的卵母细胞直径显著增加（p < ０.０５）。左旋肉碱组显示出最高的卵母细胞细胞质模式平均百分比。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记结果表明，褪黑素 + 左旋肉碱组的凋亡率最低。此外，联合组显示出最高的卵母细胞数量和成熟率。与对照组相比，所有处理组中的BMP15和GDF9基因均显著上调。

结论

我们的结果表明，联合使用褪黑素和左旋肉碱是体外促进卵母细胞成熟的更有效选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/da098dd89952/ijrb-22-527-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/6634ddf9e63d/ijrb-22-527-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/c659b3cbde81/ijrb-22-527-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/79bd11e5aa39/ijrb-22-527-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/92bf75e156f2/ijrb-22-527-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/d7ce1ccc7139/ijrb-22-527-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/da098dd89952/ijrb-22-527-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/6634ddf9e63d/ijrb-22-527-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/c659b3cbde81/ijrb-22-527-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/79bd11e5aa39/ijrb-22-527-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/92bf75e156f2/ijrb-22-527-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/d7ce1ccc7139/ijrb-22-527-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c393/11441286/da098dd89952/ijrb-22-527-g006.jpg

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