Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University , Taiyuan 030006, China.
Research Institute of Applied Chemistry, Shanxi University , Taiyuan, 030006, China.
Anal Chem. 2017 Aug 1;89(15):8079-8083. doi: 10.1021/acs.analchem.7b01580. Epub 2017 Jul 12.
Herein, a novel near-infrared fluorescent probe for ratiometric detection of bisulfate was designed and developed based on a conjugation of naphthopyran-benzothiazolium system. The sensor showed excellent selectivity, high sensitivity and a rapid response toward bisulfite in aqueous solution. Upon the addition of HSO, the sensor displayed 37-fold (I/I) fluorescence intensity enhancement, accompanied by an apparent color change from violet to colorless, suggesting that the sensor can be used to detect HSO with "naked-eye". Notably, the addition product can be applied to the design of regenerative chemodosimeters based on the HO promoted elimination of bisulfite and recovery of probe 1. Further cell and zebrafish imaging experiment demonstrated that the sensor could image the bisulfite/HO redox cycle in biological system with ratiometric manners.
本文设计并开发了一种基于萘并吡喃-苯并噻唑体系的新型近红外荧光探针，用于亚硫酸根的比率检测。该传感器在水溶液中对亚硫酸根具有优异的选择性、高灵敏度和快速响应。加入 HSO 后，传感器的荧光强度增强了 37 倍（I/I），并伴有从紫色到无色的明显颜色变化，表明该传感器可用于检测 HSO 的“裸眼”。值得注意的是，加合物可用于设计基于 HO 促进亚硫酸氢盐消除和探针 1 恢复的再生化学计量计。进一步的细胞和斑马鱼成像实验表明，该传感器可以以比率方式对生物体系中的亚硫酸氢盐/HO 氧化还原循环进行成像。
