Chen Xiaodong, Zhao Chenglu, Zhao Qiwei, Yang Yunfei, Yang Sanxiu, Zhang Rumeng, Wang Yuqing, Wang Kun, Qian Jing, Long Lingliang
Key Laboratory of Modern Agricultural Equipment and Technology (Ministry of Education), Jiangsu University, Zhenjiang 212013, China.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Foods. 2024 Jun 4;13(11):1758. doi: 10.3390/foods13111758.
Sulfites play imperative roles in food crops and food products, serving as sulfur nutrients for food crops and as food additives in various foods. It is necessary to develop an effective method for the on-site quantification of sulfites in food samples. Here, 7-(diethylamino) quinoline is used as a fluorescent group and electron donor, alongside the pyridinium salt group as an electron acceptor and the C=C bond as the sulfite-specific recognition group. We present a novel fluorescent sensor based on a mechanism that modulates the efficiency of intramolecular charge transfer (ICT), , for on-site quantitative measurement of sulfite in food. The fluorescent sensor itself exhibited fluorescence in the near-infrared light (NIR) region, effectively minimizing the interference of background fluorescence in food samples. Upon exposure to sulfite, the sensor displayed a ratiometric fluorescence response (I/I) with a high sensitivity (LOD = 0.061 μM), enabling accurate quantitative measurements in complex food environments. Moreover, sensor also displayed a colorimetric response to sulfite, making sensor measure sulfite in both fluorescence and colorimetric dual-signal modes. Sensor has been utilized for quantitatively measuring sulfite in red wine and sugar with recoveries between 99.65% and 101.90%, and the RSD was below 4.0%. The sulfite concentrations in live cells and zebrafish were also monitored via fluorescence imaging. Moreover, the sulfite assimilated by lettuce leaves was monitored, and the results demonstrated that excessive sulfite in leaf tissue could lead to leaf tissue damage. In addition, the sulfate-transformed sulfite in lettuce stem tissue was tracked, providing valuable insights for evaluating sulfur nutrients in food crops. More importantly, to accomplish the on-site quantitative measurement of sulfite in food samples, a portable sensing system was prepared. Sensor and the portable sensing system were successfully used for the on-site quantitative measurement of sulfite in food.
亚硫酸盐在粮食作物和食品中起着至关重要的作用，它既是粮食作物的硫营养物质，又是各类食品中的食品添加剂。因此，开发一种有效的食品样品中亚硫酸盐现场定量分析方法很有必要。在此，7-（二乙氨基）喹啉用作荧光基团和电子供体，吡啶鎓盐基团作为电子受体，C=C键作为亚硫酸盐特异性识别基团。我们提出了一种基于调节分子内电荷转移（ICT）效率机制的新型荧光传感器，用于食品中亚硫酸盐的现场定量测定。该荧光传感器本身在近红外（NIR）区域发出荧光，有效降低了食品样品中背景荧光的干扰。暴露于亚硫酸盐时，该传感器呈现出高灵敏度（检测限=0.061μM）的比率荧光响应（I/I），能够在复杂的食品环境中进行准确的定量测量。此外，该传感器对亚硫酸盐还呈现出比色响应，使得它能够以荧光和比色双信号模式测定亚硫酸盐。该传感器已用于定量测定红酒和糖中的亚硫酸盐，回收率在99.65%至101.90%之间，相对标准偏差低于4.0%。还通过荧光成像监测了活细胞和斑马鱼中的亚硫酸盐浓度。此外，对生菜叶吸收的亚硫酸盐进行了监测，结果表明叶片组织中过量的亚硫酸盐会导致叶片组织受损。另外，追踪了生菜茎组织中硫酸盐转化的亚硫酸盐，为评估粮食作物中的硫营养提供了有价值的见解。更重要的是，为实现食品样品中亚硫酸盐的现场定量测量，制备了一种便携式传感系统。该传感器和便携式传感系统已成功用于食品中亚硫酸盐的现场定量测量。
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