Research Unit of Analysis and Process Applied to the Environmental - APAE Higher Institute of Applied Sciences and Technology Mahdia, University of Monastir, Monastir, Tunisia; Faculty of Sciences, University of Tunis El Manar, University Campus, Tunis, Tunisia.
Catalan Institute for Water Research (ICRA), Scientific and Technological Park of The University of Girona, Girona, Spain.
Environ Pollut. 2017 Oct;229:371-374. doi: 10.1016/j.envpol.2017.05.095. Epub 2017 Jun 12.
Carbapenems are β-lactam antibiotics with a broad spectrum of activity and are usually considered the last resort for the treatment of severe infections caused by multidrug-resistant pathogens. The clinically most significant carbapenemases are KPC, NDM, and OXA-48-like enzymes, whose genes have been increasingly reported worldwide in members of the family Enterobacteriaceae. In this study, we quantified the abundance of these genes in wastewater effluents from different Tunisian hospitals. The bla and bla-like genes were detected at similar concentrations in all hospital wastewater effluents. In contrast, the bla gene was detected at lower concentration than other genes and it was only detected in three of the seven effluents analyzed. To the best of our knowledge, this study quantified for the first time the abundance of bla, bla, and bla-like genes in wastewater effluents from Tunisian hospitals, highlighting the widespread distribution of these carbapenemase genes.
碳青霉烯类抗生素是具有广谱活性的β-内酰胺类抗生素，通常被认为是治疗由多药耐药病原体引起的严重感染的最后手段。临床上最重要的碳青霉烯酶是 KPC、NDM 和 OXA-48 样酶，其基因已在世界各地的肠杆菌科成员中被越来越多地报道。在这项研究中，我们定量了来自不同突尼斯医院废水中这些基因的丰度。在所有医院废水中均以相似浓度检测到 bla 和 bla 样基因。相比之下，bla 基因的浓度低于其他基因，并且仅在分析的七个废水中的三个中检测到。据我们所知，这项研究首次定量了突尼斯医院废水中 bla、bla 和 bla 样基因的丰度，突出了这些碳青霉烯酶基因的广泛分布。
