Department of Pathophysiology, Jagiellonian University Medical College, Cracow, Poland.
Department of Immunology, Jagiellonian University Medical College, Cracow, Poland.
J Physiol Pharmacol. 2017 Apr;68(2):253-264.
Previous experiments demonstrated that low-frequency electromagnetic field (LF-EMF) may activate cellular death pathways in proliferating cells. Therefore, we hypothesized that LF-EMF may also influence viability of highly proliferating undifferentiated adipose-derived stem cells. Obesity is classified as a civilization disease; its etiopathogenesis is presumed to include both genetic predisposition and influence of modified environmental factors, such as unbalanced diet with excess calories and/or too low physical activity. Obesity may lead to a number of metabolic disorders, including type 2 diabetes mellitus, cardiovascular diseases (associated with atherosclerosis) related to primary hypertension and ischemic heart disease, myocardial infarction and other complications. The aim of this study was to verify if LF-EMF alters viability parameters of adipose-derived stem cells (ADSCs) isolated from rats, cultured in vitro and exposed to pulsed electromagnetic field (PEMF; 7 Hz, 30 mT). ADSCs were obtained from healthy rats and animals with experimentally-induced obesity, both males and females, pups and adults. The animals were fed with chow with either low (LF diet) or high fat content (HF diet) for 21 days. Then, ADSCs were isolated from extracted adipose tissue and used to establish cell cultures. ADSCs from the first passage were exposed to PEMF three times, 4 hours per exposure, at 24-h intervals (experimentally developed protocol of PEMF stimulation). 24 hours after the last exposure to PEMF, viability parameters of ADSCs were analyzed by flow cytometry (FCM). The study demonstrated that LF diet exerted a protective effect on PEMF-exposed ADSCs, especially in the case of male and female pups. In turn, the proportion of early apoptotic cells in PEMF-treated ADSC cultures from adult female rats maintained on HF diet turned out to be significantly higher than in other experimental groups.
先前的实验表明，低频电磁场（LF-EMF）可能会激活增殖细胞中的细胞死亡途径。因此，我们假设 LF-EMF 也可能影响高度增殖的未分化脂肪来源干细胞的活力。肥胖被归类为文明病；其发病机制被认为包括遗传易感性和环境因素的影响，如不平衡的饮食，摄入过多的卡路里和/或过低的身体活动。肥胖可能导致多种代谢紊乱，包括 2 型糖尿病、心血管疾病（与原发性高血压和缺血性心脏病相关）、心肌梗塞和其他并发症。本研究的目的是验证 LF-EMF 是否会改变从雄性和雌性大鼠、幼鼠和成年大鼠中分离的体外培养的脂肪来源干细胞（ADSCs）的活力参数，这些大鼠接受了脉冲电磁场（PEMF；7 Hz，30 mT）的照射。ADSCs 从健康大鼠和实验性肥胖大鼠中获得，雄性和雌性大鼠、幼鼠和成年大鼠均有。动物在 21 天内喂食低（LF 饮食）或高脂肪（HF 饮食）的饲料。然后，从提取的脂肪组织中分离 ADSCs 并用于建立细胞培养物。第一代 ADSCs 接受 PEMF 照射 3 次，每次 4 小时，间隔 24 小时（开发的 PEMF 刺激实验方案）。在最后一次暴露于 PEMF 后 24 小时，通过流式细胞术（FCM）分析 ADSCs 的活力参数。该研究表明，LF 饮食对 PEMF 暴露的 ADSCs 具有保护作用，尤其是在雄性和雌性幼鼠中。相反，在 HF 饮食组的成年雌性大鼠的 PEMF 处理的 ADSC 培养物中，早期凋亡细胞的比例明显高于其他实验组。
