意外后果：CRISPR诱导的突变导致外显子跳跃
Unexpected consequences: exon skipping caused by CRISPR-generated mutations.
作者信息
Sharpe Joshua J, Cooper Thomas A
机构信息
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, 77030, USA.
出版信息
Genome Biol. 2017 Jun 14;18(1):109. doi: 10.1186/s13059-017-1240-0.
A new study finds that splicing disruption is a frequent consequence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can express aberrant proteins. This new information allows enhanced quality control procedures to select the best mutant alleles generated by CRISPR/Cas9.Please see related Method article: https://www.doi.org/10.1186/s13059-017-1237-8.
一项新研究发现，剪接破坏是CRISPR/Cas9基因编辑技术产生的突变的常见后果，设计为无效的等位基因可能会表达异常蛋白质。这一新信息有助于加强质量控制程序，以筛选出CRISPR/Cas9产生的最佳突变等位基因。请参阅相关方法文章：https://www.doi.org/10.1186/s13059-017-1237-8 。
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