线粒体中铁硫簇的生物合成与转运
Iron-sulfur cluster biogenesis and trafficking in mitochondria.
作者信息
Braymer Joseph J, Lill Roland
机构信息
Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg.
Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg; LOEWE Zentrum für Synthetische Mikrobiologie SynMikro, Hans-Meerwein-Strasse, 35043 Marburg, Germany.
出版信息
J Biol Chem. 2017 Aug 4;292(31):12754-12763. doi: 10.1074/jbc.R117.787101. Epub 2017 Jun 14.
Abstract
The biogenesis of iron-sulfur (Fe/S) proteins in eukaryotes is a multistage, multicompartment process that is essential for a broad range of cellular functions, including genome maintenance, protein translation, energy conversion, and the antiviral response. Genetic and cell biological studies over almost 2 decades have revealed some 30 proteins involved in the synthesis of cellular [2Fe-2S] and [4Fe-4S] clusters and their incorporation into numerous apoproteins. Mechanistic aspects of Fe/S protein biogenesis continue to be elucidated by biochemical and ultrastructural investigations. Here, we review recent developments in the pursuit of constructing a comprehensive model of Fe/S protein assembly in the mitochondrion.
摘要
真核生物中铁硫（Fe/S）蛋白的生物合成是一个多阶段、多隔室的过程，对广泛的细胞功能至关重要，包括基因组维持、蛋白质翻译、能量转换和抗病毒反应。近20年来的遗传学和细胞生物学研究揭示了约30种参与细胞[2Fe-2S]和[4Fe-4S]簇合成并将其掺入众多脱辅基蛋白的蛋白质。Fe/S蛋白生物合成的机制方面仍在通过生化和超微结构研究不断阐明。在这里，我们综述了在构建线粒体中Fe/S蛋白组装综合模型方面的最新进展。
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本文引用的文献
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ACS Chem Biol. 2017 Apr 21;12(4):918-921. doi: 10.1021/acschembio.6b01005. Epub 2017 Mar 2.
2
The Relationship between Environmental Dioxygen and Iron-Sulfur Proteins Explored at the Genome Level.在基因组水平上探索环境双加氧与铁硫蛋白之间的关系。
PLoS One. 2017 Jan 30;12(1):e0171279. doi: 10.1371/journal.pone.0171279. eCollection 2017.
3
Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis.微孢子虫铁硫簇生物合成的进化保守性与体外重建。
Nat Commun. 2017 Jan 4;8:13932. doi: 10.1038/ncomms13932.
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Structure. 2016 Dec 6;24(12):2080-2091. doi: 10.1016/j.str.2016.08.020. Epub 2016 Nov 3.
5
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Metallomics. 2017 Jan 25;9(1):48-60. doi: 10.1039/c6mt00163g.
6
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9
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