Inserm U1085 IRSET, 9 Avenue du prof Léon-Bernard, 35000, Rennes, France.
EHESP, 2 Avenue du Prof Léon-Bernard, 35000, Rennes, France.
Sci Rep. 2017 Jun 14;7(1):3526. doi: 10.1038/s41598-017-03738-1.
The widely-used herbicide atrazine (ATZ) is detected in ground and surface water in many countries. Several studies in animals have demonstrated that ATZ has endocrine-disrupting effects on male and female reproduction in many vertebrate species. In this study, we investigated the effects of ATZ exposure on meiosis, a key step in gametogenesis in mammals. The treatment was initiated before oocyte entry into meiosis, which occurs during the embryonic period in females. We found that embryonic exposure to ATZ increases the level of 8-oxo-guanine in the nucleus of meiotic cells, reflecting oxidative stress and affecting meiotic double-strand break repair, chromosome synapsis and crossover numbers. Finally, embryonic exposure to ATZ reduces the number of primordial follicles and increases the incidence of multi-oocyte follicles in adult mice. Our data demonstrate that embryonic exposure to ATZ disrupts prophase I of meiosis and affects normal follicle formation in female mice.
广泛使用的除草剂莠去津（ATZ）在许多国家的地下水和地表水均有检出。多项动物研究表明，ATZ 对许多脊椎动物的雄性和雌性生殖具有内分泌干扰作用。在这项研究中，我们研究了 ATZ 暴露对减数分裂的影响，减数分裂是哺乳动物配子发生的关键步骤。该处理在卵母细胞进入减数分裂之前开始，减数分裂发生在雌性的胚胎期。我们发现，胚胎暴露于 ATZ 会增加减数分裂细胞核中 8-氧鸟嘌呤的水平，反映出氧化应激并影响减数分裂双链断裂修复、染色体联会和交叉数。最后，胚胎暴露于 ATZ 会减少原始卵泡的数量并增加成年小鼠中多卵母细胞卵泡的发生率。我们的数据表明，胚胎暴露于 ATZ 会破坏减数分裂前期 I 并影响雌性小鼠正常的卵泡形成。
