Moazeni Mohammad, Saadaty Ardakani Zahra Sadat, Saharkhiz Mohammad Jamal, Jalaei Jafar, Khademolhoseini Ali Asghar, Shams Esfand Abad Shahab, Mootabi Alavi Amir
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731 Iran.
Department of Horticulture, College of Agriculture, Shiraz University, Shiraz, Iran.
J Parasit Dis. 2017 Jun;41(2):467-472. doi: 10.1007/s12639-016-0830-1. Epub 2016 Aug 29.
seeds extract has been previously reported to have antimicrobial and other medicinal properties. The aim of this study was to evaluate the ovicidal activity of the methanolic extract of seeds against the eggs of The phenolic compounds of the methanolic extract of seeds were identified by HPLC analysis. Catechin, rutin, p-Coumaric acid, chloregenic acid and hesperetin were found to be the major phenolic compounds. eggs were collected from the gall bladder of naturally infected sheep. The eggs were exposed to two concentrations of seeds extract (1 and 3 mg/mL) for 24 and 48 h. To investigate the effect of the seeds extract on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that eggs were susceptible to the methanolic extract of seeds. Following 24 h exposure of the eggs to seeds extract with concentrations of 1 and 3 mg/mL, the miracidial formation reduced to 5 and 2.2 % respectively (compared with 60 % for the control group). Following 48 h of exposure of the eggs to seeds extract with 1 mg/mL concentration, the miracidial formation reduced to 0.5 %. In this exposure time, no miracidial formation was observed in the eggs exposed to seeds extract with concentration of 3 mg/mL. Therefore, the results of this study indicated that seeds extract has high ovicidal activity against the eggs of . Accordingly, this extract may have the potential flukicidal activity against the immature and mature .
种子提取物先前已被报道具有抗菌和其他药用特性。本研究的目的是评估种子甲醇提取物对[某种寄生虫名称]虫卵的杀卵活性。通过高效液相色谱分析鉴定了种子甲醇提取物中的酚类化合物。发现儿茶素、芦丁、对香豆酸、绿原酸和橙皮苷是主要的酚类化合物。[某种寄生虫名称]虫卵从自然感染绵羊的胆囊中收集。将虫卵暴露于两种浓度的种子提取物(1和3毫克/毫升)中24小时和48小时。为了研究种子提取物对毛蚴形成的影响,将处理过的虫卵在28℃下孵育14天。结果表明,[某种寄生虫名称]虫卵对种子甲醇提取物敏感。将虫卵暴露于浓度为1和3毫克/毫升的种子提取物24小时后,毛蚴形成分别降至5%和2.2%(对照组为60%)。将虫卵暴露于浓度为1毫克/毫升的种子提取物48小时后,毛蚴形成降至0.5%。在该暴露时间内,暴露于浓度为3毫克/毫升种子提取物的虫卵未观察到毛蚴形成。因此,本研究结果表明种子提取物对[某种寄生虫名称]虫卵具有高杀卵活性。据此,该提取物可能对未成熟和成熟的[某种寄生虫名称]具有潜在的杀吸虫活性。