Rather Hilal Ahmad, Din Mir Mehraj, Sheikh Aasif Ahmad, Tewari Anup Kumar, Maharana Biswa Ranjan
ICAR-Indian Veterinary Research Institute, Izatnagar, UP India.
Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir India.
J Parasit Dis. 2017 Jun;41(2):551-556. doi: 10.1007/s12639-016-0849-3. Epub 2016 Oct 6.
is an apicomplexan parasite capable of infecting a wide variety of warm-blooded animals, including birds and humans and is zoonotically important too. Felidae serve its definitive hosts and most infections are inoccous while in various intermediate hosts (e.g. sheep), it is responsible for abortion, still births. Humans which are immune compromised are also susceptible to toxoplasmosis. Most of the epidemiological studies have revealed it to be belonging to three clonal types with exceptions in South Africa having atypical isolates. Current genotyping was carried out at 11 genetic loci (SAG1, 5'-SAG2, 3'-SAG2, alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358 and PK1) using multiplex-nested polymerase chain reaction restriction fragment length polymorphism (Mn-PCR-RFLP). SAG1, alt SAG2, SAG3, BTUB, GRA6, C22-8, C29-2, L358 and PK1 could differentiate our strain/isolates as type I ( RH) and type III ( isolates from Chennai and Izatnagar). 5'SAG2 and 3'SAG2 in combination confirmed these as above mentioned genotypes. Further, the RH was assigned Toxo DB#10 and local isolates of were assigned Toxo DB#2. The present study is the first report on existence of Type III . lineage from animal population of Indian subcontinent based on PCR-RFLP.
是一种顶复门寄生虫，能够感染包括鸟类和人类在内的多种温血动物，并且在人畜共患病方面也很重要。猫科动物是其终末宿主，大多数感染是无症状的，而在各种中间宿主（如绵羊）中，它会导致流产、死产。免疫功能低下的人类也易患弓形虫病。大多数流行病学研究表明它属于三种克隆类型，南非存在非典型分离株的情况除外。目前使用多重巢式聚合酶链反应限制性片段长度多态性（Mn-PCR-RFLP）在11个基因位点（SAG1、5'-SAG2、3'-SAG2、替代SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358和PK1）进行基因分型。SAG1、替代SAG2、SAG3、BTUB、GRA6、C22-8、C29-2、L358和PK1可以将我们的菌株/分离株区分为I型（RH）和III型（来自金奈和伊扎特纳加尔的分离株）。5'SAG2和3'SAG2结合起来证实了这些为上述基因型。此外，RH被指定为Toxo DB#10，当地的分离株被指定为Toxo DB#2。本研究是基于PCR-RFLP首次报道印度次大陆动物种群中存在III型谱系。
