Wang Ya-Jie, Cai Shi-Jiao, Cui Jian-Lin, Chen Yang, Tang Xin, Li Yu-Hao
Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin, China.
Cataract Center, Tianjin Eye Hospital, Tianjin, China.
Neural Regen Res. 2017 May;12(5):795-803. doi: 10.4103/1673-5374.206651.
Direct exposure to intensive visible light can lead to solar retinopathy, including macular injury. The signs and symptoms include central scotoma, metamorphopsia, and decreased vision. However, there have been few studies examining retinal injury due to intensive light stimulation at the cellular level. Neural network arrangements and gene expression patterns in zebrafish photoreceptors are similar to those observed in humans, and photoreceptor injury in zebrafish can induce stem cell-based cellular regeneration. Therefore, the zebrafish retina is considered a useful model for studying photoreceptor injury in humans. In the current study, the central retinal photoreceptors of zebrafish were selectively ablated by stimulation with high-intensity light. Retinal injury, cell proliferation and regeneration of cones and rods were assessed at 1, 3 and 7 days post lesion with immunohistochemistry and hybridization. Additionally, a light/dark box test was used to assess zebrafish behavior. The results revealed that photoreceptors were regenerated by 7 days after the light-induced injury. However, the regenerated cells showed a disrupted arrangement at the lesion site. During the injury-regeneration process, the zebrafish exhibited reduced locomotor capacity, weakened phototaxis and increased movement angular velocity. These behaviors matched the morphological changes of retinal injury and regeneration in a number of ways. This study demonstrates that the zebrafish retina has a robust capacity for regeneration. Visual impairment and stress responses following high-intensity light stimulation appear to contribute to the alteration of behaviors.
直接暴露于高强度可见光会导致日光性视网膜病变，包括黄斑损伤。其体征和症状包括中心暗点、视物变形和视力下降。然而，很少有研究在细胞水平上研究强光刺激导致的视网膜损伤。斑马鱼光感受器中的神经网络排列和基因表达模式与人类相似，斑马鱼中的光感受器损伤可诱导基于干细胞的细胞再生。因此，斑马鱼视网膜被认为是研究人类光感受器损伤的有用模型。在本研究中，通过高强度光刺激选择性地消融斑马鱼的中央视网膜光感受器。在损伤后1、3和7天，用免疫组织化学和杂交技术评估视网膜损伤、视锥细胞和视杆细胞的增殖及再生情况。此外，使用明暗箱试验评估斑马鱼的行为。结果显示，光诱导损伤后7天光感受器再生。然而，再生细胞在损伤部位的排列紊乱。在损伤-再生过程中，斑马鱼的运动能力下降、趋光性减弱且运动角速度增加。这些行为在许多方面与视网膜损伤和再生的形态学变化相匹配。本研究表明，斑马鱼视网膜具有强大的再生能力。高强度光刺激后的视力损害和应激反应似乎导致了行为的改变。
