Song Cai, Yang Lei, Wang JiaJia, Chen Peirong, Li Shaomin, Liu Yingcong, Nguyen Michael, Kaluyeva Aleksandra, Kyzar Evan J, Gaikwad Siddharth, Kalueff Allan V
Research Institute and Key Laboratory for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University (GDOU), Zhanjiang, Guangdong 524025, China; Graduate Institute of Neural and Cognitive Sciences, China Medical University and Hospital, Taichung, Taiwan; The International Zebrafish Neuroscience Research Consortium (ZNRC), Slidell, LA 70458, USA.
Research Institute and Key Laboratory for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University (GDOU), Zhanjiang, Guangdong 524025, China.
Behav Brain Res. 2016 Sep 15;311:24-30. doi: 10.1016/j.bbr.2016.05.005. Epub 2016 May 4.
The zebrafish (Danio rerio) is a promising model organism for neurophenomics - a new field of neuroscience linking neural phenotypes to various genetic and environmental factors. However, the effects of prior experimental manipulations on zebrafish performance in different behavioral paradigms remain unclear. Here, we examine the influence of selected stressful procedures and test batteries on adult zebrafish anxiety-like behaviors in two commonly used models - the novel tank (NTT) and the light-dark box (LDB) tests. While no overt behavioral differences between outbred short-fin wild-type (WT) and mutant 'pink' glowfish were seen in both tests under baseline (control) conditions, an acute severe stressor (a 30-min car transportation) detected significantly lower mutant fish anxiety-like behavior in these tests. In contrast, WT zebrafish showed no overt NTT or LDB responses following a mild stressor (5-min 40-Wt light) exposure, also showing no differences in batteries of NTT and LDB run immediately one after another, or with a 1-day interval. Collectively, these findings suggest that zebrafish may be relatively less sensitive (e.g., than other popular species, such as rodents) to the test battery effect, and show that stronger stressors may be needed (to complement low-to-moderate stress aquatic screens) to better reveal phenotypical variance in zebrafish assays. Strengthening the value of zebrafish models in neurophenotyping research, this study indicates the potential of using more test batteries and a wider spectrum of pre-test stressors in zebrafish behavioral assays.
斑马鱼（Danio rerio）是神经表型组学领域一种很有前景的模式生物，神经表型组学是神经科学的一个新领域，将神经表型与各种遗传和环境因素联系起来。然而，先前的实验操作对斑马鱼在不同行为范式中的表现所产生的影响仍不清楚。在此，我们在两种常用模型——新鱼缸（NTT）和明暗箱（LDB）试验中，研究了选定的应激程序和测试组对成年斑马鱼焦虑样行为的影响。在基线（对照）条件下的两项试验中，未观察到远交短鳍野生型（WT）和突变体“粉色”荧光鱼之间有明显的行为差异，但在这些试验中，一种急性严重应激源（30分钟的汽车运输）使突变体鱼的焦虑样行为显著降低。相比之下，轻度应激源（5分钟40瓦光照）暴露后，野生型斑马鱼在NTT或LDB试验中未表现出明显反应，在紧挨着进行或间隔1天进行的NTT和LDB测试组中也未表现出差异。总体而言，这些发现表明斑马鱼可能（例如，比其他常用物种，如啮齿动物）对测试组效应相对不敏感，并且表明可能需要更强的应激源（以补充低至中度应激的水生筛选）来更好地揭示斑马鱼试验中的表型差异。本研究强化了斑马鱼模型在神经表型分型研究中的价值，表明了在斑马鱼行为试验中使用更多测试组和更广泛的测试前应激源的潜力。
