Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Korea.
Animal Planning Division, Seoul Zoo, Gwacheon, Gyenggi, Korea.
J Med Virol. 2017 Nov;89(11):1995-2002. doi: 10.1002/jmv.24875. Epub 2017 Jul 6.
Hepatitis caused by hepatitis E virus (HEV) is a public health concern worldwide. HEV strains have been isolated from several animal species, some of which induce zoonosis. Recently, the isolation of HEV from rabbits was reported. Here, the partial capsid gene (320 bp) of HEV was detected in rabbit feces via reverse transcriptase-polymerase chain reaction (RT-PCR). Rabbit HEV was found in two of six rabbit farms and 17 of 264 rabbit fecal samples (6.4%). A phylogenetic analysis of the partial capsid gene classified the 17 HEV isolates into the putative rabbit HEV clade. A full genomic sequence, KOR-Rb-1, was obtained from one rabbit HEV isolate by 5' and 3' rapid amplification of cDNA ends-PCR and RT-PCR, and comprised 7275 bp excluding the 3' poly(A) tail. It shared 77.5-86.8%, 86.6%, and 80.2-84.3% nucleotide identities with rabbit HEV isolates from China, the US, and France, respectively. It also shared 72.3-73.0%, 71.4%, 76.7-78.3%, 72.8-73.3%, and 47.1-47.2% nucleotide identities with representative strains of HEV-1, HEV-2, HEV-3, HEV-4, and avian HEV, respectively. A full-genome phylogenetic analysis classified KOR-Rb-1 into the provisional rabbit HEV clade. This isolate could be used to study the pathogenesis and zoonotic potential of rabbit HEV.
戊型肝炎病毒 (HEV) 引起的肝炎是全球公共卫生关注的问题。已经从几种动物物种中分离出 HEV 株，其中一些会引起人畜共患病。最近，从兔子中分离出 HEV 的报道。在这里，通过逆转录-聚合酶链反应 (RT-PCR) 从兔粪便中检测到 HEV 的部分衣壳基因（320bp）。在六个兔场中的两个和 264 个兔粪便样本中的 17 个（6.4%）中发现了兔 HEV。对部分衣壳基因的系统发育分析将 17 个 HEV 分离株分为假定的兔 HEV 分支。通过 5'和 3'快速扩增 cDNA 末端-PCR 和 RT-PCR 从一个兔 HEV 分离株中获得了全长基因组序列 KOR-Rb-1，全长为 7275bp，不包括 3'poly(A)尾。它与来自中国、美国和法国的兔 HEV 分离株分别共享 77.5-86.8%、86.6%和 80.2-84.3%的核苷酸同一性。它还与 HEV-1、HEV-2、HEV-3、HEV-4 和禽 HEV 的代表性株分别共享 72.3-73.0%、71.4%、76.7-78.3%、72.8-73.3%和 47.1-47.2%的核苷酸同一性。全基因组系统发育分析将 KOR-Rb-1 归入暂定的兔 HEV 分支。该分离株可用于研究兔 HEV 的发病机制和人畜共患潜力。
