通过圆二色光谱和荧光光谱检测到的tRNA与其同源氨基酸之间的特异性相互作用。

Specific interaction between tRNA and its cognate amino acid as detected by circular dichroism and fluorescence spectroscopy.

作者信息

Watanabe K, Miura K

出版信息

Biochem Biophys Res Commun. 1985 Jun 28;129(3):679-85. doi: 10.1016/0006-291x(85)91945-x.

DOI:10.1016/0006-291x(85)91945-x

Abstract

Specific interaction was detected between tRNA and its cognate L-amino acid by circular dichroism (CD) and fluorescence spectroscopy; fluorescence spectral change with saturation was observed when tRNAAsp was titrated only with L-aspartic acid, but not with D-aspartic acid, L- and D-glutamic acids. It was also the case for tRNA2Glu and L-glutamic acid as detected by CD. These results are consistent with the hypothesis that the anticodon, or the complex of four nucleotides (C4N) of the anticodon three bases and the discriminator base in a tRNA (Shimizu, M., J. Mol. Evol. (1982) 18, 297-303) participates in recognition of amino acids.

摘要

通过圆二色性（CD）和荧光光谱法检测到tRNA与其同源L-氨基酸之间存在特异性相互作用；当仅用L-天冬氨酸而非D-天冬氨酸、L-和D-谷氨酸滴定tRNA天冬氨酸时，观察到荧光光谱随饱和度的变化。通过CD检测，tRNA2谷氨酸和L-谷氨酸的情况也是如此。这些结果与以下假设一致，即反密码子或反密码子三个碱基的四个核苷酸（C4N）复合物以及tRNA中的判别碱基（Shimizu，M.，J. Mol. Evol.（1982）18，297 - 303）参与氨基酸的识别。