Li Han, Zhu Hua, Zhou Yanbing, Wang Haibo, Niu Zhaojian, Shen Yi, Lv Liang
1 Department of General Surgery, The Affiliated Hospital of Qingdao University, Qingdao, P.R. China.
2 Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Tumour Biol. 2017 Jun;39(6):1010428317705506. doi: 10.1177/1010428317705506.
Long non-coding RNAs are emerging as new players in gene regulation, but whether long non-coding RNAs influence the expression of microRNA is unclear. The expression levels of misato family member 2, pseudogene were significantly associated with lymphatic metastasis and distal metastasis in 80 paired gastric cancer tissues using real-time quantitative reverse transcription polymerase chain reaction experiments. The effects of long non-coding RNA misato family member 2, pseudogene were assessed by overexpressing or downexpressing long non-coding RNA misato family member 2, pseudogene in gastric cancer cells. Long non-coding RNA misato family member 2, pseudogene promoted gastric cancer cell growth, colony formation, migration, and invasion in gastric cancer cells. Long non-coding RNA misato family member 2, pseudogene influenced biologic functions in gastric cancer cells via indirectly regulating the activation of miR-335. Our results reveal long non-coding RNA misato family member 2, pseudogene as an oncogenic long non-coding RNA that promotes cell growth and invasion. Therefore, long non-coding RNAs might function as key regulatory hubs in gastric cancer progression.
长链非编码RNA正在成为基因调控中的新角色，但长链非编码RNA是否影响微小RNA的表达尚不清楚。运用实时定量逆转录聚合酶链反应实验，在80对胃癌组织中，假基因Misato家族成员2的表达水平与淋巴转移和远处转移显著相关。通过在胃癌细胞中过表达或下调长链非编码RNA假基因Misato家族成员2，评估其作用。长链非编码RNA假基因Misato家族成员2促进胃癌细胞生长、集落形成、迁移及侵袭。长链非编码RNA假基因Misato家族成员2通过间接调控miR-335的激活影响胃癌细胞的生物学功能。我们的结果揭示长链非编码RNA假基因Misato家族成员2是一种促进细胞生长和侵袭的致癌长链非编码RNA。因此，长链非编码RNA可能在胃癌进展中作为关键调控枢纽发挥作用。
