Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan; Vaccine Creation Project, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; BIKEN Center for Innovative Vaccine Research and Development, The Research Foundation for Microbial Diseases of Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Control Release. 2017 Aug 28;260:183-193. doi: 10.1016/j.jconrel.2017.06.007. Epub 2017 Jun 13.
Little comparative information is available on the detailed intracellular dynamics (diffusion, active movement, and distribution mechanisms) of nanoparticles (≤100nm) and sub-micron particles (>100nm). Here, we quantitatively examined the intracellular movements of different-sized particles and of the endosomal vesicles containing those particles. We showed that silica nanoparticles of various sizes (30 to 100nm) had greater motility than sub-micron particles in A549 cells. Although particles of different sizes localized in the early endosomes, late endosomes, and lysosomes in different proportions, their motilities did not vary, regardless of the vesicles in which they were localized. However, surprisingly, endosomal vesicles containing silica nanoparticles moved faster than those containing sub-micron particles. These results suggest that nanoparticles included within endosomal vesicles do not suppress the motility of the vesicles, whereas sub-micron particles perturb endosomal vesicle transport. Our data support a new hypothesis that differences in particle size influence membrane trafficking of endosomal vesicles.
关于纳米颗粒（≤100nm）和亚微米颗粒（>100nm）的详细细胞内动力学（扩散、主动运动和分布机制），相关的比较信息很少。在这里，我们定量研究了不同大小的颗粒以及包含这些颗粒的内体小泡的细胞内运动。结果表明，在 A549 细胞中，不同大小的二氧化硅纳米颗粒（30 至 100nm）比亚微米颗粒具有更大的迁移率。尽管不同大小的颗粒以不同的比例定位于早期内体、晚期内体和溶酶体中，但无论它们位于哪种小泡中，其迁移率都没有差异。然而，令人惊讶的是，包含二氧化硅纳米颗粒的内体小泡比包含亚微米颗粒的内体小泡移动得更快。这些结果表明，包含在内体小泡中的纳米颗粒不会抑制小泡的迁移，而亚微米颗粒会干扰内体小泡的运输。我们的数据支持一个新的假设，即颗粒大小的差异影响内体小泡的膜运输。
