[大鼠肝染色质非组蛋白的分离]

[Isolation of rat liver chromatin non-histone protein].

作者信息

Borshchevskaia T A, Dobretsov G E, Petrov V A

出版信息

Mol Biol (Mosk). 1975 Sep-Oct;9(5):676-82.

Abstract

A chromatin with the protein/DNA ratio of 3.0 was obtained from rat liver cells nuclei. The chromatin was dissolved in 2 M NaCl, pH 7, and reprecipitated by decreasing the ionic strength to 0.4 and increasing pH to 9.0. A fraction of non-histone proteins (NH-1) remained in a supernatant solution, the NH-1/DNA ratio being equal to 1.3 and the ratio of acidic to basic aminoacid residues equal to 1.31. After chromatography on Bio-Rex 70 cation-exchang resin, a fraction (NH-2) with the NH-2/DNA ratio of 1.0 +/- 0.15 and the ratio of acidic to basic aminoacid residues of 1.57 was obtained. According to the data of polyacrylamide gel electrophoresis, the NH-2 fraction contained 2.7% of histone f2 + f3 as an admixture whereas histone f1 was not revealed.

摘要

从大鼠肝细胞细胞核中获得了蛋白质/DNA比例为3.0的染色质。将该染色质溶解于pH 7的2M氯化钠中，通过将离子强度降低至0.4并将pH提高至9.0使其再沉淀。一部分非组蛋白（NH-1）留在上清液中，NH-1/DNA比例等于1.3，酸性与碱性氨基酸残基的比例等于1.31。在Bio-Rex 70阳离子交换树脂上进行色谱分析后，获得了NH-2/DNA比例为1.0±0.15且酸性与碱性氨基酸残基比例为1.57的一个组分（NH-2）。根据聚丙烯酰胺凝胶电泳的数据，NH-2组分含有2.7%的组蛋白f2 + f3作为杂质，而未检测到组蛋白f1。