Sudhakar Kommu, Murthy G S Sreenivasa, Rajeshwari Gaddam
Department of Veterinary Parasitology, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.
Department of Veterinary Parasitology, Teaching Veterinary Clinical Complex, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.
Vet World. 2017 May;10(5):536-541. doi: 10.14202/vetworld.2017.536-541. Epub 2017 May 21.
Bovine visceral schistosomiasis has been reported as an important disease entity as it affects animal health, productivity, causes economic losses due to liver condemnation, and produces a high morbidity. This study was conducted to standardize an easy, reliable dot-enzyme-linked immmunosorbent assay (ELISA) for the diagnosis of visceral schistosomiasis caused by Schistosoma spindale and to know the prevalence rate in and around Hyderabad.
A dot-ELISA was standardized in the laboratory using whole worm antigen (WWA) and excretory-secretory antigen (ESA) of S. spindale. The standardized test was used for the diagnosis of bovine visceral schistosomiasis at field level. The sensitivity and specificity of the test was compared with counter current immunoelectrophoresis. In total, 288 sera (125 cattle and 163 buffalo) were screened by dot-ELISA.
The dot-ELISA detected 32.63% of infection (94/288) using WWA and 40.62% of infection (117/288) using ESA. In cattle, the prevalence rate was 32.80% (41/125) using WWA and 40.80% (51/125) of infection. Similarly, in buffaloes, the prevalence rate was 32.51% (53/163) using WWA and 40.49% (66/163) of infection using ESA. The overall sensitivity of dot-ELISA was 76.74% and 80.48% with WWA and ESA, respectively, and specificity was 73.3% and 78.57% in WWA and ESA, respectively.
As ante-mortem diagnosis of visceral schistosomiasis is difficult in subclinical conditions, dot-ELISA can be used as a reliable immunodiagnostic test for diagnosis at field level.
牛内脏血吸虫病已被报道为一种重要的疾病实体，因为它影响动物健康、生产力，因肝脏废弃导致经济损失，并具有高发病率。本研究旨在标准化一种简便、可靠的斑点酶联免疫吸附测定（ELISA），用于诊断由梭形血吸虫引起的内脏血吸虫病，并了解海得拉巴及其周边地区的患病率。
在实验室中使用梭形血吸虫的全虫抗原（WWA）和排泄分泌抗原（ESA）对斑点ELISA进行标准化。标准化试验用于现场诊断牛内脏血吸虫病。将该试验的敏感性和特异性与对流免疫电泳进行比较。总共用斑点ELISA筛选了288份血清（125头牛和163头水牛）。
使用WWA时，斑点ELISA检测到32.63%的感染率（94/288），使用ESA时检测到40.62%的感染率（117/288）。在牛中，使用WWA时患病率为32.80%（41/125），感染率为40.80%（51/125）。同样，在水牛中，使用WWA时患病率为32.51%（53/163），使用ESA时感染率为40.49%（66/163）。斑点ELISA使用WWA和ESA时的总体敏感性分别为76.74%和80.48%，WWA和ESA的特异性分别为73.3%和78.57%。
由于在亚临床情况下内脏血吸虫病的生前诊断困难，斑点ELISA可作为现场诊断的可靠免疫诊断试验。
