Lakshmanan Bindu, Devada K, Joseph Siju, Binu M B, Kuttan Karthik
Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, 680-651 Kerala India.
Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, 680-651 Kerala India.
J Parasit Dis. 2016 Mar;40(1):189-93. doi: 10.1007/s12639-014-0476-9. Epub 2014 Jun 1.
Schistosomosis has been recognised as one of the major parasitic diseases of livestock and human beings. Schistosoma spindale is the major cause of visceral schistosomosis among bovines of Kerala State. Besides pathology in animals, it has been long known that cercariae of S. spindale are a common cause of dermatitis in human beings in Asia. However, detection of this disease based on coprology has underestimated the prevalence of this economically important disease among cattle of the State. An efficient diagnostic tool providing unequivocal evidence of infection in living animals is perhaps, the key to formulate and deliver control measures to the target population. It is also crucial for an enhanced understanding of parasite epidemiology. The utility of excretory-secretory proteins as diagnostic and vaccine candidates for schistosomosis has been a focus of medical research since long. There exists a paucity of information with regard to analysis of ES proteins of S. spindale and their incorporation to develop sensitive and specific serodiagnostic tool. Hence a study was designed to evaluate the efficacy of Dot-ELISA incorporating different antigens of S. spindale and to validate the test under field conditions.
血吸虫病已被公认为是人和家畜的主要寄生虫病之一。纺锤形血吸虫是喀拉拉邦牛内脏血吸虫病的主要病因。除了动物的病理学表现外，长期以来人们都知道，纺锤形血吸虫的尾蚴是亚洲人类皮炎的常见病因。然而，基于粪便学检测这种疾病低估了该邦牛群中这种具有经济重要性疾病的流行率。一种能为活体动物感染提供明确证据的有效诊断工具或许是制定并向目标人群提供控制措施的关键。这对于增强对寄生虫流行病学的理解也至关重要。长期以来，排泄分泌蛋白作为血吸虫病诊断和疫苗候选物的效用一直是医学研究的重点。关于纺锤形血吸虫ES蛋白的分析及其用于开发灵敏且特异的血清学诊断工具的信息匮乏。因此，设计了一项研究来评估采用纺锤形血吸虫不同抗原的斑点酶联免疫吸附测定（Dot-ELISA）的功效，并在田间条件下验证该检测方法。
