Department of Oral and Maxillofacial Surgery, Yamaguchi University, Graduate School of Medicine,, 1-1-1 Minamikogushi, Ube, 755-8505, Japan.
J Mater Sci Mater Med. 2017 Aug;28(8):112. doi: 10.1007/s10856-017-5913-8. Epub 2017 Jun 15.
We have already reported that the apatite coating of titanium by the blast coating (BC) method could show a higher rate of bone contact from the early stages in vivo, when compared to the pure titanium (Ti) and the apatite coating of titanium by the flame spraying (FS) method. However, the detailed mechanism by which BC resulted in satisfactory bone contact is still unknown. In the present study, we investigated the importance of various factors including cell adhesion factor in osteoblast proliferation and differentiation that could affect the osteoconductivity of the BC disks. Cell proliferation assay revealed that Saos-2 could grow fastest on BC disks, and that a spectrophotometric method using a LabAssay ALP kit showed that ALP activity was increased in cells on BC disks compared to Ti disks and FS disks. In addition, higher expression of E-cadherin and Fibronectin was observed in cells on BC disks than Ti disks and FS disks by relative qPCR as well as Western blotting. These results suggested that the expression of cell-adhesion factors, proliferation and differentiation of osteoblast might be enhanced on BC disks, which might result higher osteoconductivity.
我们已经报道过，与纯钛（Ti）和火焰喷涂（FS）法制备的钛表面的磷灰石涂层相比，爆炸喷涂（BC）法制备的钛表面的磷灰石涂层在体内的早期阶段具有更高的骨接触率。然而，BC 导致令人满意的骨接触的详细机制仍不清楚。在本研究中，我们研究了包括细胞黏附因子在内的各种因素对 BC 盘骨传导性的重要性，这些因素可能影响成骨细胞的增殖和分化。细胞增殖试验表明，Saos-2 细胞在 BC 盘上生长最快，使用 LabAssay ALP 试剂盒的分光光度法显示，BC 盘上细胞的碱性磷酸酶（ALP）活性高于 Ti 盘和 FS 盘。此外，通过相对 qPCR 和 Western blot 观察到 BC 盘上细胞的 E-钙黏蛋白和纤连蛋白的表达高于 Ti 盘和 FS 盘。这些结果表明，BC 盘上的细胞黏附因子的表达、成骨细胞的增殖和分化可能增强，这可能导致更高的骨传导性。
