Marzocco Stefania, Adesso Simona, Alilou Mostafa, Stuppner Hermann, Schwaiger Stefan
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano SA, Italy.
PhD Program in Drug Discovery and Development, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano SA, Italy.
Molecules. 2017 Jun 16;22(6):1003. doi: 10.3390/molecules22061003.
Jacq., Asteraceae, is a plant which is used in traditional alpine medicine. Historical sources describe the medical use of the root, but up until now only a few studies evaluated its pharmacological properties. The evaluation of the dichloromethane extract, and its major compounds for their anti-inflammatory and anti-oxidant potential was performed in macrophages J774A.1 and C6 astrocytes. Nitric oxide (NO) and reactive oxygen species (ROS) release, as well as nitrotyrosine formation, were evaluated. Moreover, in order to evaluate the potential anti-proliferative activity, under the same experimental conditions, 3-(4,5-dimethyltiazol-2yl)-2,5-phenyl-2-tetrazolium bromide (MTT) assay was also performed. Our results indicate that has a significant effect in inhibiting both pro-inflammatory and pro-oxidative mediators. All isolated compounds were able to significantly inhibit NO and ROS release both in macrophage and in astrocytes cells, even if the effect was more pronounced in macrophage. In particular, among the tested compounds, 6,12-dihydroxy-(-)-2-tremetone exerted stronger activity. Both extract and single compounds did not affect cellular viability. This study provides evidence for the pharmacological anti-inflammatory and anti-oxidant potential of extract. These effects could be due to the activity of its major constituents and subsequent identification of benzofurans as a promising compound class to combat inflammation and related diseases.
雅克（菊科）是一种用于传统高山医学的植物。历史资料记载了其根部的药用价值，但截至目前，仅有少数研究评估过其药理特性。在巨噬细胞J774A.1和C6星形胶质细胞中对二氯甲烷提取物及其主要化合物的抗炎和抗氧化潜力进行了评估。评估了一氧化氮（NO）和活性氧（ROS）的释放以及硝基酪氨酸的形成。此外，为了评估潜在的抗增殖活性，在相同实验条件下还进行了3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2-四氮唑溴盐（MTT）测定。我们的结果表明，[提取物名称未给出]在抑制促炎和促氧化介质方面具有显著作用。所有分离出的化合物均能显著抑制巨噬细胞和星形胶质细胞中NO和ROS的释放，尽管在巨噬细胞中的作用更为明显。特别是，在所测试的化合物中，6,12-二羟基-(-)-2-震颤酮表现出更强的活性。提取物和单一化合物均未影响细胞活力。本研究为[提取物名称未给出]提取物的药理抗炎和抗氧化潜力提供了证据。这些作用可能归因于其主要成分的活性，随后鉴定出苯并呋喃是对抗炎症及相关疾病的有前景的化合物类别。
