费雷蒂亚树（FERETIA APODANTHERA）根皮提取物的植物化学分析、抗氧化和抗炎潜力
Phytochemical analysis, antioxidant and anti-inflammatory potential of FERETIA APODANTHERA root bark extracts.
作者信息
Owolabi Oluwayinka Olufunmilayo, James Dorcas Bolanle, Sani Ibrahim, Andongma Binda T, Fasanya Opeoluwa O, Kure Barnabas
机构信息
Bioresources Development Centre, National Biotechnology Development Agency, Ya'adua way, Lugbe, Airport Road, Abuja, FCT, Nigeria.
Biochemistry Department, Faculty of Science, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
出版信息
BMC Complement Altern Med. 2018 Jan 12;18(1):12. doi: 10.1186/s12906-017-2070-z.
BACKGROUND
Inflammation has been implicated in many disorders, including cancer and available therapies elicit adverse effects. Plants of the family Rubiaceae have shown potency against inflammation. The anti-inflammatory and anti-oxidant potential of Feretia apodanthera was investigated in this study to evaluate its effectiveness.
METHODS
The phytochemical, antioxidant and anti-inflammatory potential of root bark (n-Hexane, diethyl ether, ethanol and aqueous) extracts of Feretia apodanthera was investigated in this study. The extracts were subjected to various chemical tests for phytochemical constituents; their antioxidant activity was determined using in-vitro DPPH radical scavenging activity assay and their anti-inflammatory activity was determined using carrageenan induced paw oedema model. FTIR and GCMS analysis was done to determine the compounds present.
RESULTS
Phytochemical screening of extracts revealed the presence of unsaturated steroids, triterpenes, cardiac glycosides, tannins, saponin and alkaloids. Vitamin C had a median inhibitory concentration (IC) of 0.038 mg/ml which was lower than IC of all the extracts. Of all the extracts, ethanol extract had the lowest IC (0.044 mg/ml) which is comparable to vitamin C. Anti-inflammatory studies showed that the inflammation inhibition potential of 400 mg/kg body weight of all the extracts was significantly lower (p < 0.05) than the standard ketoprofen (50 mg/kg) at the first three hours but significantly higher (p < 0.05) at the fourth hour. At the fifth hour, the inflammation inhibition potential of diethyl ether, ethanol and aqueous extracts were significantly higher (p < 0.05) than that of the standard. FTIR analysis showed the presence of ketones, amines, alkenes and carboxylic groups. GCMS analysis revealed compounds that are potential anti-inflammatory agents.
CONCLUSION
This study revealed that extracts of Feretia apodanthera possess anti-inflammatory effects against right hind paw oedema of albino rats and can act as an effective antioxidant.
背景
炎症与包括癌症在内的多种疾病有关，且现有疗法会引发不良反应。茜草科植物已显示出抗炎效力。本研究对阿波丹瑟费雷蒂亚（Feretia apodanthera）的抗炎和抗氧化潜力进行了研究，以评估其有效性。
方法
本研究对阿波丹瑟费雷蒂亚根皮（正己烷、乙醚、乙醇和水）提取物的植物化学、抗氧化和抗炎潜力进行了研究。提取物接受了各种植物化学成分的化学测试；使用体外DPPH自由基清除活性测定法测定其抗氧化活性，并使用角叉菜胶诱导的爪肿胀模型测定其抗炎活性。进行了傅里叶变换红外光谱（FTIR）和气相色谱 - 质谱联用（GCMS）分析以确定存在的化合物。
结果
提取物的植物化学筛选显示存在不饱和甾体、三萜类、强心苷、单宁、皂苷和生物碱。维生素C的半数抑制浓度（IC）为0.038毫克/毫升，低于所有提取物的IC。在所有提取物中，乙醇提取物的IC最低（0.044毫克/毫升），与维生素C相当。抗炎研究表明，体重400毫克/千克的所有提取物在前三个小时的炎症抑制潜力明显低于标准酮洛芬（50毫克/千克）（p < 0.05），但在第四小时显著更高（p < 0.05）。在第五小时，乙醚、乙醇和水提取物的炎症抑制潜力显著高于标准品（p < 0.05）。FTIR分析显示存在酮、胺、烯烃和羧基。GCMS分析揭示了潜在的抗炎剂化合物。
结论
本研究表明，阿波丹瑟费雷蒂亚提取物对白化大鼠右后爪肿胀具有抗炎作用，并且可以作为一种有效的抗氧化剂。
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