Li Siwei, Maçon Anthony L B, Jacquemin Manon, Stevens Molly M, Jones Julian R
1 Department of Materials, Imperial College London, London, UK.
2 Department of Bioengineering, Imperial College London, London, UK.
J Biomater Appl. 2017 Jul;32(1):104-113. doi: 10.1177/0885328217706640.
Wnt-signalling cascade is one of the crucial pathways involved in the development and homeostasis of cartilage. Influencing this pathway can potentially contribute to improved cartilage repair or regeneration. One key molecular regulator of the Wnt pathway is the glycogen synthase kinase-3 enzyme, the inhibition of which allows initiation of the signalling pathway. This study aims to utilise a binary SiO-LiO sol-gel derived glass for controlled delivery of lithium, a known glycogen synthase kinase-3 antagonist. The effect of the dissolution products of the glass on chondrogenic differentiation in an in vitro 3D pellet culture model is reported. Dissolution products that contained 5 mM lithium and 3.5 mM silicon were capable of inducing chondrogenic differentiation and hyaline cartilaginous matrix formation without the presence of growth factors such as TGF-β3. The results suggest that sol-gel derived glass has the potential to be used as a delivery vehicle for therapeutic lithium ions in cartilage regeneration applications.
Wnt信号级联是参与软骨发育和内环境稳定的关键途径之一。影响该途径可能有助于改善软骨修复或再生。Wnt途径的一个关键分子调节因子是糖原合酶激酶-3酶，对其抑制可启动信号通路。本研究旨在利用二元SiO-LiO溶胶-凝胶衍生玻璃来控制锂（一种已知的糖原合酶激酶-3拮抗剂）的递送。报道了该玻璃溶解产物对体外三维微球培养模型中软骨形成分化的影响。含有5 mM锂和3.5 mM硅的溶解产物能够在不存在诸如转化生长因子-β3等生长因子的情况下诱导软骨形成分化和透明软骨基质形成。结果表明，溶胶-凝胶衍生玻璃有潜力用作软骨再生应用中治疗性锂离子的递送载体。
