一种适用于转运研究的兔空肠分离肠上皮细胞制剂。
A rabbit jejunal isolated enterocyte preparation suitable for transport studies.
作者信息
Brown P D, Sepúlveda F V
出版信息
J Physiol. 1985 Jun;363:257-70. doi: 10.1113/jphysiol.1985.sp015708.
Abstract
A method is described for isolating viable enterocytes from rabbit jejunum. Estimates of sucrase and gamma-glutamyl transferase activities in cells isolated by this method suggest that they originate from the upper villus only. Isolated cells accumulate both alpha-methyl-D-glucoside and alanine, maintaining high intracellular concentrations for at least 60 and 40 min respectively. Accumulation of alpha-methyl-D-glucoside is inhibited by the presence of phloridzin. The cells accumulate 42K and 86Rb in an identical manner. This uptake, which is maintained for at least 60 min, is inhibited in the presence of ouabain. Passive efflux of 42K and 86Rb occurs with rate constants which are virtually identical. The efflux follows a single exponential suggesting that it originates from only one intracellular compartment. It is suggested that the preparation can be used to study the effect of sugars and amino acids on K efflux. The advantages of using such a preparation are discussed.
摘要
本文描述了一种从兔空肠中分离活肠上皮细胞的方法。用该方法分离的细胞中蔗糖酶和γ-谷氨酰转移酶活性的估计表明，它们仅起源于绒毛上部。分离的细胞积累α-甲基-D-葡萄糖苷和丙氨酸，分别在至少60分钟和40分钟内保持高细胞内浓度。根皮苷的存在会抑制α-甲基-D-葡萄糖苷的积累。细胞以相同的方式积累⁴²K和⁸⁶Rb。这种摄取至少维持60分钟，在哇巴因存在时会受到抑制。⁴²K和⁸⁶Rb的被动外流以几乎相同的速率常数发生。外流遵循单一指数，表明它仅起源于一个细胞内区室。建议该制剂可用于研究糖和氨基酸对钾外流的影响。讨论了使用这种制剂的优点。
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