Brown P D, Burton K A, Sepúlveda F V
FEBS Lett. 1983 Nov 14;163(2):203-6. doi: 10.1016/0014-5793(83)80819-9.
A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium (86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with 86Rb and the rate constant of efflux was seen to increase when actively transported sugars or amino acids are added to the bathing medium. The increase is related to the transport of the non-electrolyte, but not to volume regulatory events.
描述了一种使用透明质酸酶从兔空肠中分离上皮细胞的技术。所获得的细胞保留了逆浓度梯度积累糖类和钾（86Rb）的能力。使用预先加载86Rb的细胞监测钾外流，当向浴液中添加主动转运的糖类或氨基酸时，外流速率常数会增加。这种增加与非电解质的转运有关，而与体积调节事件无关。
