Kimmich G A, Randles J
Am J Physiol. 1981 Nov;241(5):C227-32. doi: 10.1152/ajpcell.1981.241.5.C227.
The unidirectional influx of alpha-methylglucoside (alpha-MG) by isolated chicken intestinal epithelial cells is 98% inhibited by phlorizin. The remaining 2% of the total influx occurs in the absence of Na+, is not sensitive to phloretin, and is equal to the diffusional entry rate for 2-deoxyglucose. The glucoside is much more strongly accumulated (75-fold) than 3-O-methylglucose (3-OMG) (10-fold). Inhibitors of the serosal sugar carrier (phloretin, cytochalasin B, theophylline, and flavanoids) do not enhance alpha-MG accumulation. It is concluded that the glycoside is not a substrate for the intestinal serosal transport system. Steady-state gradients of the sugar can be represented accurately by a concentrative, phlorizin-sensitive system that is opposed by a diffusional efflux process.
分离出的鸡肠上皮细胞对α-甲基葡萄糖苷（α-MG）的单向内流被根皮苷抑制了98%。总内流中剩余的2%在没有钠离子的情况下发生，对根皮素不敏感，且与2-脱氧葡萄糖的扩散进入速率相等。该糖苷比3-O-甲基葡萄糖（3-OMG）积累得更强（75倍）（3-OMG为10倍）。浆膜糖载体抑制剂（根皮素、细胞松弛素B、茶碱和类黄酮）不会增强α-MG的积累。得出的结论是，该糖苷不是肠浆膜转运系统的底物。糖的稳态梯度可以由一个浓缩的、对根皮苷敏感的系统准确表示，该系统与扩散外流过程相反。
