Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University (BHU), Varanasi, 221005, India.
ETH Zurich, Institute of Integrative Biology, Forest Pathology and Dendrology, 8092, Zurich, Switzerland.
Sci Rep. 2017 Jun 16;7(1):3745. doi: 10.1038/s41598-017-03933-0.
Fungal endophytes were isolated from leaf, bark and stem of Tectona grandis Linn.f. sampled at four geographical locations in winter, summer and monsoon seasons. The recovered 5089 isolates were assigned to 45 distinct morphotypes based on morphology. The sequences of the internal transcribed spacers (ITS) of the nrDNA of some morphotypes were identical, but morphological differences were strong enough to consider these morphotypes as separate species. Forty-three morphotypes were assigned to ascomycotina and two to basidiomycotina. Ascomycotina was the predominating group with 99.7% of total isolates followed by basidiomycotina with only 0.3% of total isolates. Diaporthe (Phomopsis) species dominated the communities independently on tissue type, location or season. More than 60% of the examined tissue pieces were colonized by members of this species complex. While these endophytes are ubiquitous others were tissue or location specific. Tissue type had the strongest effect on the species evenness of the endophytic assemblage followed by geographical location and season. However, Shannon-Wiener index (H') significantly (p ≤ 0.001) varied with all three factors i.e. season, location and tissue type. Leaves supported the highest diversity across all the seasons and locations. In conclusion, all the three factors together determined the structure of endophytic mycobiota assemblage of T. grandis.
在冬季、夏季和季风季节，从四个地理位置采集的柚木（Tectona grandis Linn.f.）的叶、皮和茎中分离出真菌内生菌。回收的 5089 个分离物根据形态分为 45 个不同的形态型。一些形态型的 nrDNA 内转录间隔区（ITS）序列相同，但形态差异足够大，足以将这些形态型视为独立的物种。43 个形态型被分配到子囊菌门，2 个被分配到担子菌门。子囊菌门是主要群体，占总分离物的 99.7%，其次是担子菌门，仅占总分离物的 0.3%。Diaporthe（Phomopsis）物种独立于组织类型、位置或季节主导群落。超过 60%的检查组织块被该物种复合体的成员定殖。虽然这些内生菌无处不在，但其他内生菌是组织或位置特异性的。组织类型对内生菌组合的物种均匀度影响最大，其次是地理位置和季节。然而，香农-威纳指数（H'）显著（p≤0.001）随季节、位置和组织类型这三个因素而变化。叶片在所有季节和地点都支持最高的多样性。总之，这三个因素共同决定了柚木内生真菌菌群的结构。
