Heinrich-Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
Institute of Virology, University of Veterinary Medicine, Hannover, Germany.
Sci Rep. 2017 Jun 16;7(1):3734. doi: 10.1038/s41598-017-03997-y.
We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recovered from a skin lesion of a grey seal thereby preventing sequence changes due to in vitro passaging of the virus. Subsequent analysis of the proteins encoded by this virus identified genes specific for skin adaptation and pathogenesis of parapoxviruses. These data warrant the classification of seal parapoxvirus, tentatively designated SePPV, as a new species within the genus Parapoxvirus.
我们使用第二代短读长测序技术和第三代纳米孔测序技术相结合，直接从复杂的临床标本中恢复了全长痘病毒基因组，包括末端发夹区。我们直接从一只灰海豹的皮肤病变中直接恢复了完整的病毒基因组序列，从而防止了因病毒体外传代而导致的序列变化。对该病毒编码的蛋白质进行的后续分析鉴定出了一些特定的基因，这些基因与副痘病毒的皮肤适应和发病机制有关。这些数据证明，海豹副痘病毒（暂定名为 SePPV）有理由被分类为副痘病毒属中的一个新种。
