坦桑尼亚家猪中非洲猪瘟病毒基因型 II、IX 和 XV 的全基因组分析。

Complete genome analysis of African swine fever virus genotypes II, IX and XV from domestic pigs in Tanzania.

机构信息

OR Tambo Africa Research Chair for Viral Epidemics, SACIDS Foundation for One Health, Sokoine University of Agriculture, PO Box 3297, Morogoro, Tanzania.

Department of Biosciences, Solomon Mahlangu College of Natural and Applied Sciences, Sokoine University of Agriculture, PO Box 3038, Morogoro, Tanzania.

出版信息

Sci Rep. 2023 Mar 31;13(1):5318. doi: 10.1038/s41598-023-32625-1.

DOI:10.1038/s41598-023-32625-1

PMID:37002287

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10066019/

Abstract

African swine fever (ASF) caused by ASF virus (ASFV) is an infectious transboundary animal disease notifiable to the World Organization for Animal Health causing high mortality in domestic pigs and wild boars threatening the global domestic pig industry. To date, twenty-four ASFV genotypes have been described and currently genotypes II, IX, X, XV and XVI are known to be circulating in Tanzania. Despite the endemic status of ASF in Tanzania, only one complete genome of ASFV from the country has been described. This study describes the first complete genome sequence of ASFV genotype XV. In addition, the first Tanzanian complete genome of ASFV genotype IX and three ASFV strains belonging to genotype II collected during ASF outbreaks in domestic pigs in Tanzania were determined in this study using Illumina sequencing and comparative genomics analysis. The generated ASFV complete genome sequences ranged from 171,004 to 184,521 base pairs in length with an average GC content of 38.53% and encoded 152 to 187 open reading frames. The results of this study provide insights into the genomic structure of ASFV and can be used to monitor changes within the ASFV genome and improve our understanding of ASF transmission dynamics.

摘要

非洲猪瘟（ASF）由 ASF 病毒（ASFV）引起，是一种传染性的跨界动物疾病，需向世界动物卫生组织报告，它会导致家猪和野猪的高死亡率，威胁着全球的家猪产业。迄今为止，已经描述了二十四种 ASFV 基因型，目前已知在坦桑尼亚流行的基因型有 II、IX、X、XV 和 XVI。尽管 ASF 在坦桑尼亚呈地方性流行，但该国仅描述了一种 ASFV 的完整基因组。本研究描述了 ASFV 基因型 XV 的第一个完整基因组序列。此外，本研究还使用 Illumina 测序和比较基因组学分析，确定了坦桑尼亚首例 ASFV 基因型 IX 的完整基因组和在坦桑尼亚家猪 ASF 暴发期间收集的三种属于基因型 II 的 ASFV 毒株。生成的 ASFV 完整基因组序列长度在 171,004 到 184,521 个碱基之间，平均 GC 含量为 38.53%，编码 152 到 187 个开放阅读框。本研究的结果提供了对 ASFV 基因组结构的深入了解，并可用于监测 ASFV 基因组内的变化，提高我们对 ASF 传播动态的理解。