Turk J Med Sci. 2015;45(1):27-32.
BACKGROUND/AIM: TO examine the effects of royal jelly (RJ) on testicular damage in streptozotocin (STZ)-induced diabetic rats.
Eighteen adult Wistar albino rats were used, 6 in each of the 3 treatment groups: Group A: control, Group B: STZ-induced diabetes (untreated), Group C: STZ-induced diabetes plus RJ (400 mg/kg daily for 4 weeks). Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg). Four weeks after the onset of diabetes, testicular apoptotic cell death was examined using immunohistochemical staining for caspase-3 and Ki67 staining for localization of proliferative cells.
Compared with the control, the body and testicular weights of the RJ-treated and untreated diabetic rats were decreased (P < 0.05). The histopathological examination showed a significant increase in degenerative changes in the seminiferous tubules and in spermatogenesis of the STZ-treated rats. In contrast, the RJ treatment group showed near-normal morphology, in addition to an increased intensity of immunohistochemical staining for Ki67-positive cells.
Diabetes induced a significant increase in testicular apoptotic cell death (caspase-3-positive cells). Caspase-3-positive cells were significantly decreased in the STZ plus RJ-treated group compared with the untreated STZ-induced diabetic group (P < 0.05).
背景/目的：研究蜂王浆（RJ）对链脲佐菌素（STZ）诱导的糖尿病大鼠睾丸损伤的影响。
使用 18 只成年 Wistar 白化大鼠，每组 6 只，分为 3 组：A 组：对照组，B 组：STZ 诱导的糖尿病（未治疗），C 组：STZ 诱导的糖尿病加 RJ（400mg/kg 每日 4 周）。糖尿病通过单次腹腔注射 STZ（60mg/kg）诱导。糖尿病发病 4 周后，通过 caspase-3 免疫组织化学染色和 Ki67 染色定位增殖细胞来检查睾丸细胞凋亡。
与对照组相比，RJ 治疗组和未治疗的糖尿病大鼠的体重和睾丸重量均降低（P<0.05）。组织病理学检查显示 STZ 处理组的曲细精管退行性变化和精子发生明显增加。相比之下，RJ 治疗组表现出接近正常的形态，此外，Ki67 阳性细胞的免疫组织化学染色强度增加。
糖尿病导致睾丸细胞凋亡（caspase-3 阳性细胞）显著增加。与未治疗的 STZ 诱导的糖尿病组相比，STZ 加 RJ 治疗组的 caspase-3 阳性细胞明显减少（P<0.05）。
