Lin Lin, Xu Weili, Zhang Guojian, Ren Pengtao, Zhao Jing, Yan Qinghui
Department of Colorectal Anal Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Department of Colorectal Anal Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Immunol Lett. 2017 Aug;188:59-63. doi: 10.1016/j.imlet.2017.06.007. Epub 2017 Jun 15.
Interleukin-22 (IL-22), an IL-10 family cytokine produced by T cells and innate lymphoid cells, is implicated in inflammation and tumorigenesis. In this study, we aimed to investigate the association of IL-22 polymorphisms with the colon cancer in a Chinese population.
Five hundred forty colon cancer cases and 540 healthy controls were recruited in the case-control study. The fluorogenic 5' exonuclease assays were used for genotype analysis of three common polymorphisms (-429C/T, +1046T/A and +1995A/C) of the IL-22 gene.
Colon cancer cases had a significantly higher frequency of IL-22-429 TT genotype [odds ratio (OR)=1.69, 95% confidence interval (CI)=1.24, 2.30; P=0.001] and -429T allele (OR=1.35, 95% CI=1.14, 1.60; P=0.001) than healthy controls. The findings are still emphatic by the Bonferroni correction (P<0.017). When stratifying by the differentiation of colon cancer, we found that colon cancer cases with poor differentiation had a significantly higher frequency of IL-22-429 TT genotype (OR=1.45, 95% CI=1.02, 2.07; P=0.04). When stratifying by the tumor location, tumor size, growth pattern and TNM stage of colon cancer, we found no statistical association. The IL-22 +1046T/A and IL-22 +1995A/C gene polymorphisms were not associated with colon cancer.
Our findings suggested that the IL-22 -429C/T gene polymorphisms might be associated with colon cancer.
白细胞介素-22（IL-22）是一种由T细胞和固有淋巴细胞产生的IL-10家族细胞因子，与炎症和肿瘤发生有关。在本研究中，我们旨在调查中国人群中IL-22基因多态性与结肠癌的关联。
在病例对照研究中招募了540例结肠癌病例和540名健康对照。采用荧光5'核酸外切酶分析法对IL-22基因的三种常见多态性（-429C/T、+1046T/A和+1995A/C）进行基因分型分析。
与健康对照相比，结肠癌病例中IL-22 -429 TT基因型[比值比（OR）=1.69，95%置信区间（CI）=1.24，2.30；P=0.001]和-429T等位基因（OR=1.35，95%CI=1.14，1.60；P=0.001）的频率显著更高。经Bonferroni校正后，结果仍然显著（P<0.017）。按结肠癌分化程度分层时，我们发现低分化结肠癌病例中IL-22 -429 TT基因型的频率显著更高（OR=1.45，95%CI=1.02，2.07；P=0.04）。按结肠癌的肿瘤位置、肿瘤大小、生长方式和TNM分期分层时，我们未发现统计学关联。IL-22 +1046T/A和IL-22 +1995A/C基因多态性与结肠癌无关。
我们的研究结果表明，IL-22 -429C/T基因多态性可能与结肠癌有关。
