Department of Gynecology, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qing Chun Road, Hangzhou, Zhejiang 310003, PR China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, Zhejiang 310058, PR China; Dr.Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regeneration Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, PR China.
Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, Zhejiang 310058, PR China; Dr.Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regeneration Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, PR China.
Stem Cell Reports. 2017 Jul 11;9(1):381-396. doi: 10.1016/j.stemcr.2017.05.022. Epub 2017 Jun 15.
The endometrial layer comprises luminal and glandular epithelia that both develop from the same simple layer of fetal uterine epithelium. Mechanisms of uterine epithelial progenitor self-renewal and differentiation are unclear. This study aims to systematically analyze the molecular and cellular mechanisms of uterine epithelial development by single-cell analysis. An integrated set of single-cell transcriptomic data of uterine epithelial progenitors and their differentiated progenies is provided. Additionally the unique molecular signatures of these cells, characterized by sequential upregulation of specific epigenetic and metabolic activities, and activation of unique signaling pathways and transcription factors, were also investigated. Finally a unique subpopulation of early progenitor, as well as differentiated luminal and glandular lineages, were identified. A complex cellular hierarchy of uterine epithelial development was thus delineated. Our study therefore systematically decoded molecular markers and a cellular program of uterine epithelial development that sheds light on uterine developmental biology.
子宫内膜层由腔上皮和腺上皮组成，它们均由胎儿子宫上皮的同一单层发育而来。子宫上皮祖细胞自我更新和分化的机制尚不清楚。本研究旨在通过单细胞分析系统地分析子宫上皮发育的分子和细胞机制。提供了一组子宫上皮祖细胞及其分化后代的单细胞转录组数据集。此外，还研究了这些细胞的独特分子特征，其特征是特定表观遗传和代谢活性的顺序上调，以及独特信号通路和转录因子的激活。最后，鉴定了早期祖细胞的一个独特亚群以及分化的腔上皮和腺谱系。因此，描绘了一个复杂的子宫上皮发育细胞层次结构。我们的研究因此系统地解码了子宫上皮发育的分子标记和细胞程序，为子宫发育生物学提供了新的见解。
