动态干细胞异质性

Dynamic stem cell heterogeneity.

作者信息

Krieger Teresa, Simons Benjamin D

机构信息

The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK Cavendish Laboratory, Department of Physics, J. J. Thomson Avenue, University of Cambridge, Cambridge CB3 0HE, UK Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK

出版信息

Development. 2015 Apr 15;142(8):1396-406. doi: 10.1242/dev.101063.

DOI:10.1242/dev.101063

PMID:25852198

Abstract

Recent lineage-tracing studies based on inducible genetic labelling have emphasized a crucial role for stochasticity in the maintenance and regeneration of cycling adult tissues. These studies have revealed that stem cells are frequently lost through differentiation and that this is compensated for by the duplication of neighbours, leading to the consolidation of clonal diversity. Through the combination of long-term lineage-tracing assays with short-term in vivo live imaging, the cellular basis of this stochastic stem cell loss and replacement has begun to be resolved. With a focus on mammalian spermatogenesis, intestinal maintenance and the hair cycle, we review the role of dynamic heterogeneity in the regulation of adult stem cell populations.

摘要

最近基于诱导性基因标记的谱系追踪研究强调了随机性在成年循环组织维持和再生中的关键作用。这些研究表明，干细胞经常通过分化而丢失，而这通过相邻细胞的复制得到补偿，从而导致克隆多样性的巩固。通过将长期谱系追踪分析与短期体内实时成像相结合，这种随机性干细胞丢失和替代的细胞基础已开始得到解决。我们重点关注哺乳动物的精子发生、肠道维持和毛发周期，综述动态异质性在成年干细胞群体调控中的作用。