Lehmann Sylvia G, Bourgoin-Voillard Sandrine, Seve Michel, Rachidi Walid
University Grenoble Alpes, ISTerre, CS 40700, 38058 Grenoble Cedex 9, France.
University Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, Grenoble, France.
Oxid Med Cell Longev. 2017;2017:5140360. doi: 10.1155/2017/5140360. Epub 2017 May 23.
Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.
皮肤老化是一个复杂的过程，人们已经做出了很多努力来确定有助于诊断、预防和治疗皮肤老化的新的特定靶点。几项关于皮肤老化的研究分析了基因表达的变化，而在蛋白质水平上进行的研究很少。此外，这些蛋白质组学研究均未使用全局定量标记蛋白质组非凝胶方法，该方法能够更准确地描述老化表型。我们将这种方法应用于从年轻和老年女性未暴露于阳光的皮肤活检中获得的人原代角质形成细胞。共鉴定出517种独特的蛋白质，其中58种蛋白质有显著差异表达，随着年龄增长，40种下调，18种上调。对这58种假定的皮肤老化生物标志物进行基因本体论和通路分析表明，这些失调的蛋白质大多参与代谢和细胞过程，如细胞周期和信号通路。通过对来自多个供体的样本进行蛋白质印迹法，证实了微管蛋白β-3链表达的变化。因此，本研究表明微管蛋白β-3链在皮肤老化中是一种有前景的生物标志物。
