人A1腺苷受体的光亲和标记及质谱交联位置分析
Photoaffinity Labeling of the Human A Adenosine Receptor and Cross-link Position Analysis by Mass Spectrometry.
作者信息
Muranaka Hideyuki, Momose Takaki, Handa Chiaki, Ozawa Tomonaga
机构信息
Central Research Laboratories, Kissei Pharmaceutical Co., Ltd., 4365-1 Kashiwabara, Hotaka, Azumino, Nagano 399-8304, Japan.
出版信息
ACS Med Chem Lett. 2017 May 17;8(6):660-665. doi: 10.1021/acsmedchemlett.7b00138. eCollection 2017 Jun 8.
Abstract
Photoaffinity labeling (PAL) is widely used for the identification of ligand-binding proteins and elucidation of ligand-binding sites. PAL has also been employed for the characterization of G protein-coupled receptors (GPCRs); however, a limited number of reports has successfully identified their cross-linked amino acids. This report is the first of its kind to determine the cross-link position of the human A adenosine receptor by PAL with the novel diazirine-based photoaffinity probe .
摘要
光亲和标记（PAL）被广泛用于鉴定配体结合蛋白和阐明配体结合位点。PAL也已用于G蛋白偶联受体（GPCR）的表征；然而，成功鉴定其交联氨基酸的报道数量有限。本报告首次通过使用新型基于重氮环丙烷的光亲和探针，利用PAL确定人A腺苷受体的交联位置。
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