Sharma Arun, Rao Vepa Kameswara, Kamboj Dev Vrat, Gaur Ritu, Upadhyay Sanjay, Shaik Mahabul
Defence Research and Development Establishment, Gwalior, M.P. 474002, India.
Biotechnol Rep (Amst). 2015 Feb 20;6:129-136. doi: 10.1016/j.btre.2015.02.004. eCollection 2015 Jun.
In this paper an attempt was made to detect B (SEB) both by electrochemical and fluorescence immunoassay methods using zinc sulphide (ZnS) QDs. Wet-chemical method was adopted for the preparation of fluorescent ZnS QDs (diameter ∼ 5-10 nm). These QDs were bioconjugated with monoclonal antibodies and then characterized by various method. A detection limit of 0.02 ng mL by fluorescence assay and 1.0 ng mL by electrochemical assay for SEB was achieved. While by sandwich ELISA it is possible to detect 0.24 ng mL only. The sensitivity of all techniques is very good, since the LD of SEB is 20 ng kg. Electrochemical assay is faster, need low-cost instrument, independent to the size of QDs and found to be one of the best alternative methods as compared to the other existing methods studied herein. The presented method could be expanded to the development of electrochemical and fluorescence biosensors for various agents for field and laboratory use.
本文尝试使用硫化锌（ZnS）量子点，通过电化学和荧光免疫分析方法检测葡萄球菌肠毒素B（SEB）。采用湿化学法制备荧光ZnS量子点（直径约5 - 10纳米）。这些量子点与单克隆抗体进行生物共轭，然后通过各种方法进行表征。荧光分析法对SEB的检测限为0.02纳克/毫升，电化学分析法的检测限为1.0纳克/毫升。而夹心酶联免疫吸附测定法仅能检测到0.24纳克/毫升。所有技术的灵敏度都非常好，因为SEB的致死剂量为20纳克/千克。电化学分析法速度更快，所需仪器成本低，与量子点大小无关，并且与本文研究的其他现有方法相比，是最佳替代方法之一。所提出的方法可扩展用于开发适用于现场和实验室使用的针对各种试剂的电化学和荧光生物传感器。
