Ruan Yao, Xu Huanhuan, Yu Jinlu, Chen Qian, Gu Lihong, Guo Ailing
National Research and Development Center for Egg Processing, Huazhong Agriculture University Wuhan 430070 Hubei Province China
Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education Wuhan 430070 China
RSC Adv. 2019 Dec 11;10(2):1042-1049. doi: 10.1039/c9ra08651j. eCollection 2020 Jan 2.
A fluorescence immunoassay has been widely applied in different fields due to its high sensitivity, simple operations, and high accuracy. Quantum dots (QDs) are often selected as labels in a fluorescence immunoassay due to their high fluorescence, better stability, and biocompatibility. In this study, novel doped CdTe : Zn/ZnS QDs with stability and a high photoluminescence quantum yield (40.78%) were prepared by the water synthesis method and used as labels to conjugate with goat anti-rabbit IgG to establish a fluorescence immunoassay (FLISA) for bacteria compared to the traditional enzyme-linked immunosorbent assay (ELISA) based on the reaction between an antibody and an antigen. A good linear relationship between the fluorescence intensity and concentrations of CM13 was found when the concentrations were in the range of 10 CFU mL-10 CFU mL. The limit of detection (LOD) of CM13 was 1.25 × 10 CFU mL by FLISA, which was about 80 times lower than the LOD obtained from ELISA (10 CFU mL). This indicated that our FLISA method has higher sensitivity than traditional ELISA, and the CdTe : Zn/ZnS QDs synthesized in this paper have good applications in the rapid sensitive detection of microorganisms.
荧光免疫分析因其高灵敏度、操作简单和高精度而在不同领域得到广泛应用。量子点（QDs）由于其高荧光性、更好的稳定性和生物相容性，常被选作荧光免疫分析中的标记物。在本研究中，通过水相合成法制备了具有稳定性和高光致发光量子产率（40.78%）的新型掺杂CdTe : Zn/ZnS量子点，并将其用作标记物与山羊抗兔IgG偶联，与基于抗体和抗原反应的传统酶联免疫吸附测定（ELISA）相比，建立了一种用于细菌检测的荧光免疫分析（FLISA）。当CM13浓度在10 CFU/mL至10 CFU/mL范围内时，发现荧光强度与CM13浓度之间具有良好的线性关系。通过FLISA测定，CM13的检测限（LOD）为1.25×10 CFU/mL，比ELISA获得的检测限（10 CFU/mL）低约80倍。这表明我们的FLISA方法比传统ELISA具有更高的灵敏度，并且本文合成的CdTe : Zn/ZnS量子点在微生物的快速灵敏检测中具有良好的应用前景。
