Hammes Thais Ortiz, Leke Renata, Escobar Thayssa Dalla Costa, Fracasso Laisa Beduschi, Meyer Fabiola Schons, Andrades Michael Éverton, Silveira Themis Reverbel da
Hospital de Clínicas de Porto Alegre. Experimental Research Center. Experimental Laboratory of Hepatology and Gastroenterology. Porto Alegre, RS, Brazil..
Nutr Hosp. 2017 Jun 5;34(3):702-709. doi: 10.20960/nh.626.
The intestinal dysbiosis is common in chronic liver disease and can induce to inflammatory responses and mediate the collagen deposition in the liver.
To evaluate the probiotic Lactobacillus rhamnosus GG (LGG) for the treatment of liver fibrosis in a model of chronic cholestatic liver disease in rats.
Male adult Wistar rats (n = 29) were submitted to common bile duct ligation (BDL groups) or manipulation of common bile duct without ligation (Ctrl groups).Two weeks after surgery, each group was randomly divided to receive 1 ml of PBS (Ctrl and BDL) or PBS containing 2.5 x 107 CFU of LGG (Ctrl-P and BDL-P) through gavages for 14 days. Euthanasia occurred 33 days after surgery when samples of blood and liver tissue were collected.
The hepatic gene expression of Tlr4, Tnfα, IL-6, Tgfβ, and metalloproteinase-2 and -9 were higher in the BDL groups in comparison to Ctrl. The ductular reaction evaluated by immunocontent of cytokeratin-7 (CK7) and the content of collagen were increased in BDL groups. Also, there was an imbalance in the antioxidant defenses (superoxide dismutase and catalase) and an increase in the oxidative stress marker sulfhydryl in BDL groups. The treatment with LGG significantly reduced gene expression of IL-6, collagen deposition, and ductular reaction in hepatic tissue of animals from BDL-P groups.
The treatment with the probiotic LGG was able to reduce liver fibrosis, ductular reaction, and hepatic gene expression of IL-6 in a model of cholestatic liver disease in rats.
肠道菌群失调在慢性肝病中很常见，可诱发炎症反应并介导肝脏中的胶原蛋白沉积。
在大鼠慢性胆汁淤积性肝病模型中评估益生菌鼠李糖乳杆菌GG（LGG）对肝纤维化的治疗作用。
将成年雄性Wistar大鼠（n = 29）进行胆总管结扎（BDL组）或不结扎胆总管的操作（对照组）。手术后两周，每组随机分为通过灌胃接受1 ml PBS（对照组和BDL组）或含2.5×10⁷ CFU LGG的PBS（对照组-P和BDL-P），持续14天。术后33天处死大鼠，采集血液和肝组织样本。
与对照组相比，BDL组中Tlr4、Tnfα、IL-6、Tgfβ以及金属蛋白酶-2和-9的肝脏基因表达更高。通过细胞角蛋白-7（CK7）免疫含量评估的胆管反应以及BDL组中的胶原蛋白含量增加。此外，BDL组中抗氧化防御（超氧化物歧化酶和过氧化氢酶）失衡，氧化应激标志物巯基增加。LGG治疗显著降低了BDL-P组动物肝组织中IL-6的基因表达、胶原蛋白沉积和胆管反应。
在大鼠胆汁淤积性肝病模型中，益生菌LGG治疗能够减轻肝纤维化、胆管反应以及肝脏中IL-6的基因表达。
