Center of Super-Diamond and Advanced Films (COSDAF) & Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China.
Nanotechnology. 2017 Jul 14;28(28):285102. doi: 10.1088/1361-6528/aa744c. Epub 2017 Jun 19.
Polymer dots (Pdots) have emerged as a new type of fluorescent probe material for biomedical applications and have attracted great interest due to their excellent optical properties and biocompatability. In this work, we report on a red-emitting PHT Pdot fluorescent probe for intracellular bioimaging. The as-prepared Pdot fluorescent probe exhibits good stability and has a large Stokes shift (121 nm) compared to molecules in tetrahydrofuran (THF). Furthermore, the probe shows low cytotoxicity, broad absorption spectrum, resistance against photodegradation, and good water dispersibility. These advantageous characteristics make PHT Pdots a promising fluorescent probe material for bioimaging.
聚合物点（Pdots）作为一种新型的荧光探针材料，在生物医学应用中引起了极大的关注，因为它们具有优异的光学性质和生物相容性。在这项工作中，我们报道了一种用于细胞内生物成像的红色发射 PHT Pdot 荧光探针。所制备的 Pdot 荧光探针表现出良好的稳定性，与四氢呋喃（THF）中的分子相比，具有较大的斯托克斯位移（121nm）。此外，该探针显示出低细胞毒性、宽吸收光谱、抗光降解性和良好的水分散性。这些有利的特性使得 PHT Pdots 成为生物成像有前途的荧光探针材料。
