Department of Chemistry , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.
Department of Bioengineering , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.
Anal Chem. 2019 Aug 20;91(16):10357-10360. doi: 10.1021/acs.analchem.9b02300. Epub 2019 Jul 30.
This letter describes formation of single chain cationic polymer dots (Pdots) made of poly[1,4-dimethyl-1-(3-((2,4,5-trimethylthiophen-3-yl)oxy)propyl)piperazin-1-ium bromide] conjugated polyelectrolyte (CPE). The single chain Pdot formation relies on a simple process which is a rapid nanophase separation between CPE solution of ethylene glycol and water. Pdots show narrow monodisperse size distribution with a 3.6 nm in diameter exhibiting high brightness and excellent colloidal and optical stability. It has been demonstrated that photoluminescent Pdots provide selective nuclear translocation to hepatocellular carcinoma cells as compared to healthy liver cells. The Pdot labeling effectively discriminates cancer cells in the coculture media. Pdots hold great promise as a luminescent probe to diagnose cancer cells in histology and may guide surgeons during operations to precisely separate out cancerous tissue due to augmented fluorescence brightness.
这封信描述了由聚[1,4-二甲基-1-(3-((2,4,5-三甲基噻吩-3-基)氧基)丙基)哌嗪-1-鎓溴化物]共轭聚电解质(CPE)制成的单链阳离子聚合物点(Pdots)的形成。单链 Pdot 的形成依赖于一种简单的过程，即 CPE 在乙二醇和水之间的快速纳米相分离。Pdots 表现出窄的单分散尺寸分布，直径为 3.6nm，具有高亮度和优异的胶体和光学稳定性。已经证明，与健康的肝细胞相比，发光 Pdots 提供了对肝癌细胞的选择性核转位。与共培养培养基中的健康细胞相比，Pdot 标记能够有效地将癌细胞与健康细胞区分开来。由于荧光亮度增强，Pdots 有望成为一种用于组织学中诊断癌细胞的发光探针，并可能在手术过程中指导外科医生精确地分离癌组织。
