Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USA.
Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO 65211, USA.
Sensors (Basel). 2022 Sep 23;22(19):7218. doi: 10.3390/s22197218.
Semiconducting polymer dots (Pdots) are rapidly becoming one of the most studied nanoparticles in fluorescence bioimaging and sensing. Their small size, high brightness, and resistance to photobleaching make them one of the most attractive fluorophores for fluorescence imaging and sensing applications. This paper highlights our recent advances in fluorescence bioimaging and sensing with nanoscale luminescent Pdots, specifically the use of organic dyes as dopant molecules to modify the optical properties of Pdots to enable deep red and near infrared fluorescence bioimaging applications and to impart sensitivity of dye doped Pdots towards selected analytes. Building on our earlier work, we report the formation of secondary antibody-conjugated Pdots and provide Cryo-TEM evidence for their formation. We demonstrate the selective targeting of the antibody-conjugated Pdots to FLAG-tagged FLS2 membrane receptors in genetically engineered plant leaf cells. We also report the formation of a new class of luminescent Pdots with emission wavelengths of around 1000 nm. Finally, we demonstrate the formation and utility of oxygen sensing Pdots in aqueous media.
半导体聚合物点（Pdots）迅速成为荧光生物成像和传感领域研究最多的纳米粒子之一。其小尺寸、高亮度和抗光漂白性使它们成为最有吸引力的荧光团之一，适用于荧光成像和传感应用。本文重点介绍了我们在纳米级发光 Pdots 的荧光生物成像和传感方面的最新进展，特别是使用有机染料作为掺杂分子来修饰 Pdots 的光学性质，以实现深红光和近红外荧光生物成像应用，并赋予掺杂染料的 Pdots 对选定分析物的敏感性。基于我们早期的工作，我们报告了形成了与二级抗体缀合的 Pdots，并提供了 Cryo-TEM 证据证明其形成。我们证明了抗体缀合的 Pdots 对遗传工程植物叶片细胞中 FLAG 标记的 FLS2 膜受体的选择性靶向。我们还报告了发射波长约为 1000nm 的一类新的发光 Pdots 的形成。最后，我们证明了在水介质中形成和利用氧传感 Pdots 的情况。
