Shenzhen Second People's Hospital, Clinical Medicine College of Anhui Medical University, Shenzhen, Guangdong 518039, P.R. China.
Department of Urinary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518037, P.R. China.
Oncol Rep. 2017 Aug;38(2):917-925. doi: 10.3892/or.2017.5711. Epub 2017 Jun 12.
Stromal antigen 2 (STAG2) is a subunit of the cohesion complex that plays an important role in the normal segregation of sister chromatids during mitosis or meiosis. However, the effect of STAG2 on the bladder cancer cell proliferation, migration, and invasion has not yet been fully clarified. In this study, we aimed to characterize STAG2 expression and functional significance in BC and adjacent normal tissue. Notably, STAG2 expression was markedly lower in BC cells and tumor tissues than their normal counterparts at the gene and protein levels. Moreover, clinicopathological analysis showed that the low STAG2 expression is associated with TNM stage. Functional analysis demonstrated that STAG2 overexpression attenuated cell proliferation via G1-phase arrest, invasion, and migration, and promoted apoptosis in BC cell lines, while the opposite was observed with STAG2 knockdown cells. Furthermore, STAG2 overexpression upregulated E-cadherin, caspase-3, and caspase-7 and downregulated vimentin, matrix metalloproteinase (MMP)2, and MMP9. Collectively, these data suggest that STAG2 acts as a tumor suppressor gene in bladder cancer and may be a potential therapeutic target in BC.
基质抗原 2（STAG2）是黏合复合物的一个亚基，在有丝分裂或减数分裂过程中姐妹染色单体的正常分离中发挥重要作用。然而，STAG2 对膀胱癌细胞增殖、迁移和侵袭的影响尚未完全阐明。在本研究中，我们旨在研究 STAG2 在膀胱癌及癌旁正常组织中的表达及其功能意义。值得注意的是，与正常对照相比，STAG2 在膀胱癌细胞和肿瘤组织中的基因和蛋白水平均明显下调。此外，临床病理分析表明，低表达 STAG2 与 TNM 分期相关。功能分析表明，STAG2 过表达通过 G1 期阻滞、侵袭和迁移抑制细胞增殖，并促进膀胱癌细胞系的凋亡，而 STAG2 敲低细胞则观察到相反的结果。此外，STAG2 过表达上调 E-钙黏蛋白、半胱天冬酶-3 和半胱天冬酶-7，下调波形蛋白、基质金属蛋白酶（MMP）2 和 MMP9。综上所述，这些数据表明 STAG2 在膀胱癌中起抑癌基因的作用，可能是膀胱癌的潜在治疗靶点。
