Department of Neurosurgery, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Oncol Rep. 2017 Aug;38(2):1156-1162. doi: 10.3892/or.2017.5728. Epub 2017 Jun 16.
miR-30a has been found to be dysregulated in diverse cancers and involved in the regulation of tumor progression. However, there is scarce research on the role of miR-30a in glioma. In the present study, we assessed the expression level of miR-30a in glioma tissues and cell lines. The microRNA microarray analysis revealed low expression of miR-30a in glioma tissues and cells vs. the control. Furthermore, we found that stable miR-30a inhibited cell proliferation, G1 phase arrest and stem cell-like formation in glioma. Moreover, to investigate the molecular mechanism of miR-30a on glioma cell phenotypes, we identified Wnt5a as a new direct target gene for miR-30a by bioinformatic assay, luciferase assay and western blot analysis. Further functional studies suggested that miR-30a suppressed metastasis, sphere formation and glioma growth by targeting Wnt5a signal pathway. Collectively, our findings suggested for the first time that miR-30a may function as a tumor suppressor in glioma by targeting Wnt5a.
miR-30a 在多种癌症中被发现失调，并参与肿瘤进展的调控。然而，miR-30a 在神经胶质瘤中的作用研究甚少。在本研究中，我们评估了 miR-30a 在神经胶质瘤组织和细胞系中的表达水平。miRNA 微阵列分析显示，与对照相比，miR-30a 在神经胶质瘤组织和细胞中表达下调。此外，我们发现稳定表达的 miR-30a 可抑制神经胶质瘤细胞的增殖、G1 期阻滞和干细胞样形成。此外，为了研究 miR-30a 对神经胶质瘤细胞表型的分子机制，我们通过生物信息学分析、荧光素酶报告基因检测和 Western blot 分析鉴定出 Wnt5a 是 miR-30a 的一个新的直接靶基因。进一步的功能研究表明，miR-30a 通过靶向 Wnt5a 信号通路抑制转移、球体形成和神经胶质瘤生长。综上所述，我们的研究结果首次表明，miR-30a 可能通过靶向 Wnt5a 发挥肿瘤抑制作用，参与神经胶质瘤的发生发展。
