Sevcik Emily N, Szymanski John M, Jallerat Quentin, Feinberg Adam W
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania.
Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania.
Curr Protoc Cell Biol. 2017 Jun 19;75:10.23.1-10.23.25. doi: 10.1002/cpcb.25.
The cell microenvironment plays an important role in many biological processes, including development and disease progression. Key to this is the extracellular matrix (ECM), a complex biopolymer network serving as the primary insoluble signaling network for physical, chemical, and mechanical cues. In vitro, the ability to engineer the ECM at the micro- and nanoscales is a critical tool to systematically interrogate the influence of ECM properties on cellular responses. Specifically, both topographical and chemical surface patterning has been shown to direct cell alignment and tissue architecture on biomaterial surfaces, however, it has proven challenging to independently control these surface properties. This protocol describes a method termed Patterning on Topography (PoT) to engineer 2D nanopatterns of ECM proteins onto topographically complex substrates, which enables independent control of physical and chemical surface properties. Applications include interrogation of fundamental cell-surface interactions and engineering interfaces that can direct cell and/or tissue function. © 2017 by John Wiley & Sons, Inc.
细胞微环境在包括发育和疾病进展在内的许多生物学过程中发挥着重要作用。关键在于细胞外基质（ECM），它是一个复杂的生物聚合物网络，作为物理、化学和机械信号的主要不溶性信号网络。在体外，在微米和纳米尺度上构建细胞外基质的能力是系统研究细胞外基质特性对细胞反应影响的关键工具。具体而言，地形和化学表面图案化已被证明可引导生物材料表面上的细胞排列和组织结构，然而，独立控制这些表面特性已被证明具有挑战性。本方案描述了一种称为地形图案化（PoT）的方法，用于在地形复杂的底物上构建细胞外基质蛋白的二维纳米图案，从而能够独立控制物理和化学表面特性。应用包括研究基本的细胞-表面相互作用以及设计能够引导细胞和/或组织功能的界面。© 2017约翰威立国际出版公司
