Gantner Pierre, Velay Aurelie, Guigue Nicolas, Barth Heidi, Wendling Marie-Josee, Delaugerre Constance, Fafi-Kremer Samira
Clin Lab. 2017 May 1;63(5):997-1001. doi: 10.7754/Clin.Lab.2017.161026.
Human T-cell lymphotropic virus type 1 and 2 (HTLV-1/2) immunoassays are used for blood screen- ing from blood products, milk, and organ donors.
We assessed the performance of the DiaSorin Liaison® XL murex recHTLV-I/II immunoassay relative to the Abbott Architect® rHTLV-I/II immunoassay and with the Innogenetics immunoblot as confirmation.
A panel of HTLV positive (n = 66) and negative (n = 30) sera was tested in both techniques within the same freeze/thaw cycle. The specificity and sensitivity of DiaSorin immunoassay were 100% and 78.8%, respectively. Abbott and DiaSorin immunoassays showed a correlation in chemiluminiscent signals to cutoff (S/CO) (Pearson r = 0.92). Half of the samples (34/66) from the seropositive panel were not confirmed by immunoblot (S/CO < 5 in both techniques).
Our data confirmed that the DiaSorin Liaison® XL murex recHTLV-I/II immunoassay is an effective platform for HTLV screening. Due to false-positive reaction, especially for samples with low S/CO, each seropositive sample should be confirmed by immunoblot.
人类嗜T淋巴细胞病毒1型和2型（HTLV-1/2）免疫测定法用于对血液制品、牛奶和器官捐献者的血液进行筛查。
我们评估了DiaSorin Liaison® XL murex recHTLV-I/II免疫测定法相对于雅培Architect® rHTLV-I/II免疫测定法的性能，并以内基因免疫印迹法作为确认方法。
在同一冻融循环中，用这两种技术对一组HTLV阳性（n = 66）和阴性（n = 30）血清进行了检测。DiaSorin免疫测定法的特异性和敏感性分别为100%和78.8%。雅培和DiaSorin免疫测定法在化学发光信号与临界值（S/CO）方面显示出相关性（Pearson r = 0.92）。血清阳性组的一半样本（34/66）未通过免疫印迹法确认（两种技术的S/CO均<5）。
我们的数据证实，DiaSorin Liaison® XL murex recHTLV-I/II免疫测定法是一种有效的HTLV筛查平台。由于存在假阳性反应，尤其是对于S/CO较低的样本，每个血清阳性样本都应通过免疫印迹法进行确认。
