Rochat Laurence, Croxatto Antony, De Vallière Serge, D'Acremont Valérie, Genton Blaise
Centre de vaccination et médecine des voyages, Policlinique médicale universitaire, CHUV, 1011 Lausanne.
Laboratoire de microbiologie, CHUV, 1011 Lausanne.
Rev Med Suisse. 2017 May 3;13(561):963-967.
Until recently, the search for enteropathogens causing travellers' diarrhea was based on stool culture (Campylobacter spp., Salmonella spp. and Shigella spp.), direct microscopy with (Cryptosporidium spp.) or without specific staining (Giardia lamblia, Entamoeba histolytica) or specific antigen detection (Giardia lamblia, Entamoeba histolytica). Molecular analyses are progressively replacing traditional diagnostic methods but their clinical usefulness remains to be better defined. This article attempts to describe the advantages and disadvantages of these new molecular methods and to illustrate situations where they could be useful using clinical cases frequently encountered in the practice of travel medicine.
直到最近，对导致旅行者腹泻的肠道病原体的检测仍基于粪便培养（弯曲杆菌属、沙门氏菌属和志贺氏菌属）、直接显微镜检查（隐孢子虫属）或不进行特殊染色（蓝氏贾第鞭毛虫、溶组织内阿米巴）或特定抗原检测（蓝氏贾第鞭毛虫、溶组织内阿米巴）。分子分析正逐渐取代传统诊断方法，但其临床实用性仍有待进一步明确。本文试图描述这些新分子方法的优缺点，并通过旅行医学实践中经常遇到的临床病例来说明它们可能有用的情况。
